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Ventana image viewer software

Manufactured by Roche
Sourced in Germany

The Ventana Image Viewer software is a tool developed by Roche for viewing and analyzing digital pathology images. The software allows users to access, navigate, and manipulate high-resolution digital images of tissue samples. It provides basic image viewing and annotation capabilities.

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4 protocols using ventana image viewer software

1

Formalin Fixation and Histological Assessment of Lung Tissue

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Whole lungs were inflated with 10% formalin (Fisher Scientific) to preserve pulmonary architecture. Lungs were processed, paraffin embedded, sectioned (4-5 μm) and stained with hematoxylin and eosin by the UNMC Tissue Sciences Core Facility. Slides were scanned using the Ventana HT iScan (Roche Diagnostic, Mannheim Germany) and images acquired (20 x) using the Ventana Image Viewer software (Roche). Slides were reviewed and semi-quantitatively assessed (assigned a score from 0 to 5, with a higher score indicting greater inflammatory changes) by a veterinary pathologist blinded to treatment conditions.
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2

Caspase-3 Expression in FFPE Lung Sections

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FFPE lung sections were stained for caspase-3 expression by the UNMC Tissue Sciences Core Facility using standardized protocols. Briefly, slides were stained with caspase-3 primary antibody (Abcam, Cambridge, MA), washed, incubated with anti-HRP antibody, washed, stained with H2O2 and DAB ChromoMap (Roche), and counterstained with hematoxylin. Slides were scanned using the Ventana HT iScan (Roche) and images acquired (40 x) using the Ventana Image Viewer software (Roche).
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3

Quantifying GPR18 Immunoreactivity in Tissue

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Tissue slides with at least 100 cells were selected to further quantify the percentage (0%–100%) and intensity (low vs. medium vs. high) of GPR18 immunoreactivity, resulting in ten digital images for the analyses.
VSM and EVT were annotated (digitally marked) in ten digital images using VENTANA Image Viewer software (version 3.1.3; Roche, Indianapolis, IN, USA), a web-based application by BioImagene.
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4

Formalin-fixed Lung Tissue Histology

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Whole lungs were inflated with 10% formalin (Thermo Fisher Scientific) to preserve pulmonary architecture. Lungs were processed, paraffin embedded, sectioned (4–5 µm), and stained with hematoxylin and eosin by the UNMC Tissue Sciences Core Facility. Slides were scanned using the Ventana HT iScan (Roche Diagnostic, Mannheim Germany) and images were acquired (20×) using the Ventana Image Viewer software (Roche). Slides were reviewed and semi-quantitatively assessed (assigned a score from 0 to 5, with a higher score indicting greater inflammatory changes) by a pathologist (TAW) blinded to the treatment conditions.
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