Reduced l gsh

Manufactured by Merck Group

Sourced in United States

Reduced l-GSH is a laboratory product manufactured by Merck Group. It is a chemical compound consisting of the amino acids glycine, cysteine, and glutamic acid. Reduced l-GSH serves as a cofactor in various enzymatic reactions and plays a role in cellular antioxidant mechanisms.

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L-glutathione reduced (GSH) (50 citations) Reduced GSH (41 citations) L-GSH (8 citations)

5 protocols using reduced l gsh

Preparation of Polymeric Nanoparticles for Drug Delivery

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Acrylamide (A9099, ≥99%), N,N′-bis(acryloyl)cystamine (A4929), ammonium persulfate (A3678, ≥98%), N,N,N′,N′-tetramethylethylenediamine (T9281, 99%), sodium alginate (A2033, medium viscosity), calcium sulfate (C3771, ≥99%), methyl blue (M6900), barium sulfate (11844), L-GSH reduced (≥98%), EDTA (≥99%), DMSO (D8418), sodium bicarbonate (NaHCO₃, S5761), calcium carbonate (CaCO₃, ≥99%), and phosphate-buffered saline (PBS, pH 7.4) were available commercially from Sigma-Aldrich and used as received unless otherwise noted. Insulin was kindly provided by Novo Nordisk and labeled by Alexa-Fluor® 488. Lumefantrine and rifampicin were purchased from Hangzhou Hysen Pharma Co., Ltd in China. Nanopure water (18 MΩ cm) was acquired by means of a Milli-Q water filtration system, Millipore (St. Charles). SGF (pH ~1.2) was made by dissolving 2 g NaCl and 8.3 ml concentrated HCL in nanopure water and adjusting to 1000 ml.

Liu J., Pang Y., Zhang S., Cleveland C., Yin X., Booth L., Lin J., Lucy Lee Y.A., Mazdiyasni H., Saxton S., Kirtane A.R., Erlach T.V., Rogner J., Langer R, & Traverso G. (2017). Triggerable tough hydrogels for gastric resident dosage forms. Nature Communications, 8, 124.

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Advanced Glycation End-Product Detection

Cited 1 time

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L-GSH (reduced) (cat# G6429), hydrogen chloride in dry ethanol (cat# 17934), cystamine dihydrochloride (cat# C121509), methylglyoxal (cat# 67028), erythrulose (cat# 56845), ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA) (cat# E5134), copper chloride (cat# 10125-13-0), L-ascorbic acid (cat# A7631), Chelex-100 (cat# C7901), O-phenylenediamine (OPD) (cat# 694975), and 1H-pyrazole-1-carboxamidine HCl (cat# 402516) were purchased from Sigma-Aldrich (St. Louis, MO). Amberlite IRA 402 (OHˉ form) (cat# A18185) was obtained from Alfa Aesar (Tewksbury, MA). CEL, CML, GOLD, GOLA, MODIC and pentosidine were synthesized as previously described [37 (link)–42 (link)]. Antibody against αAC (cat# ADI-SPA-221-F) was obtained from Enzo Life Sciences (Farmingdale, NY). αBC antibody (cat# ABN185) was purchased from Millipore Sigma (Burlington, MA). Antibodies for β-crystallin (β-C) (cat# SC22745) and γ-crystallin (γ-C) (cat# SC22746) were obtained from Santa Cruz Biotechnology (Dallas, TX). HRP-conjugated anti-rabbit IgG (cat# 7074S) was purchased from Cell Signaling Technology (Danvers, MA). All other chemicals used were of analytical grade.

Nandi S.K., Rankenberg J., Rakete S., Nahomi R.B., Glomb M.A., Linetsky M.D, & Nagaraj R.H. (2020). Glycation-mediated protein crosslinking and stiffening in mouse lenses are inhibited by carboxitin in vitro. Glycoconjugate journal, 38(3), 347-359.

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Monoclonal Antibody Modification Protocol

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The monoclonal antibodies including anti–4-1BB (anti-CD137, clone 3H3), anti–PD-1 (clone 29F.1A12), anti–CTLA-4 (anti-CD152, clone 9H10), rat IgG2a isotype control (anti-TNP, clone 2A3), and anti-CD3 (clone 17A2) were purchased from Bio X Cell (West Lebanon, NH, USA). Methoxy PEG amine (mPEG-NH₂) with various MWs, methoxy PEG thiol (mPEG-SH), and methoxy PEG–DBCO (mPEG-DBCO) were purchased from JenKem Technology (Plano, TX, USA). Alexa Fluor 647 succinimidyl ester was purchased from Invitrogen (now Thermo Fisher Scientific, Waltham, MA, USA). Benzoyl peroxide [BPO; containing 25% (w/w) water as stabilizer, CAS 94-36-0] was purchased from Alfa Aesar (Ward Hill, MA, USA) and recrystallized twice with chloroform and methanol before use. 2-Hydroxyethyl disulfide (technical grade, CAS 1892-29-1), phosgene [15% (w/w) in toluene, CAS 75-44-5], trimethylamine (TEA; 99.5%, CAS 121-44-8), NHS (98%, CAS 6066-82-6), dimethylmaleic anhydride (DMMA; 98%, CAS 766-39-2), N-bromosuccinimide (NBS; 99%, CAS 128-08-5), TNBSA [5% (w/v) in H₂O, CAS 2508-19-2], SDS (20% in H₂O, CAS 151-21-3), reduced l-GSH (98%, CAS 70-18-8), and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Unless otherwise noted, all chemicals and reagents were used as received.

Zhao Y., Xie Y.Q., Van Herck S., Nassiri S., Gao M., Guo Y, & Tang L. (2021). Switchable immune modulator for tumor-specific activation of anticancer immunity. Science Advances, 7(37), eabg7291.

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Chemical Screen for Cell Death Modulators

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The following drugs and chemicals were used in this study: JQ1 (11187), Bortezomib (BTZ, 10008822), Liproxstatin‐1 (17730), rotenone (13995) and carbonyl cyanide m‐chlorophenyl hydrazine (CCCP, 25458) were purchased from Cayman Chemicals. SP600125 (1496) was from Tocris Bioscience. Imidazole ketone erastin (IKE) was from Taizhou Crene Biotechnology, China. The following were purchased from Sigma: Ferrostatin‐1 (SML0583), zVAD‐FMK (V116), Necrostatin‐1 (N9037), reduced l‐GSH (G6013), 2,2′‐bipyridyl (D216305), staurosporine (S5921), Doxicycline (D9891, D3072) bafilomycin‐A1 (B1793), α‐ketoglutarate (K1128), erastin (E7781), RSL3 (SML2234), FIN56 (SML1740), aminooxyacetic acid (AOA, C13408), pioglitazone (CDS021593), tunicamycin (T7765), paclitaxel (T7402) and chloroquine (C6628).

Vinik Y., Maimon A., Dubey V., Raj H., Abramovitch I., Malitsky S., Itkin M., Ma'ayan A., Westermann F., Gottlieb E., Ruppin E, & Lev S. (2024). Programming a Ferroptosis‐to‐Apoptosis Transition Landscape Revealed Ferroptosis Biomarkers and Repressors for Cancer Therapy. Advanced Science, 11(17), 2307263.

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Antioxidant and Diabetic Assays

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Reduced L-GSH and Alloxan were obtained from Sigma-Aldrich Inc. (Sigma-Aldrich, St. Louis, MO, USA). Dibasic sodium phosphate and glucose were obtained from ADWIC (CAIRO, Egypt). Yeast extract, agar-agar and peptone were obtained from SAS chemical Co., (MUMBAI, India) . Malt extract, Glycine, L-Cysteine and L-Glutamic acid were bought from LOBA Chemie (MUMBAI, India). All solutions were prepared using deionized distilled water.

Badr H., El-Baz A., Mohamed I., Shetaia Y., El-Sayed A.S.A, & Sorour N. (2021). Bioprocess optimization of glutathione production by Saccharomyces boulardii: biochemical characterization of glutathione peroxidase. Archives of microbiology, 203(10).

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