Tc s 7001

RND3 overexpression and knockdown, ROCK1 knockdown and ROCK2 knockdown were achieved by lentiviral transfection. Lentiviral particles for gene manipulation were purchased from Cyagen Biosciences (Suzhou, China) and were applied following manufacturer's instructions. For reducing the miR-182-5p or miR-96-5p expression level, cells at 50% confluence on a 24 well plate were treated with antagomir targeting miR-182-5p or miR-96-5p (GenePharma, Shanghai, China) at 50 nM for 24 hours in the presence of 1% (v/v) Lipofectamine 3000 (Thermo Fisher Scientific). To mimic the overexpression of miR-182-5p or miR-96-5p, cells were treated with agomir under the same conditions as that for antagomir treatment. Non-targeting agomir and antagomir controls (agomir NC and antagomir NC, respectively, GenePharma) were used as negative controls for agomir and antagomir treatment, respectively. To inhibit ROCK1/2 kinase activity, cells were treated with TC-S 7001 (Tocris, Bio-techne, Shanghai, China) at 10 nM for 24 hours. Treatment with cisplatin (Tocris) was performed at 30 μg mL⁻¹ together with other treatments, as indicated earlier.