Fluorescent imaging of HECs on the patterned substrates was performed using a fully apochromatic corrected stereomicroscope with 12.5∶1 zoom (M125, Leica). Images were acquired with a 10X objective lens, binning of 4×4, gain of 8.0, and brightness of 1.2 was used for image acquisition. Stained cells were imaged using a Leica SP5 confocal microscope. Spot Imaging Software and Leica SP5 LAS Software were used for image acquisition, and images were processed using ImageJ (v1.46, NIH).
Spot imaging software
Manufactured by Leica
Spot Imaging Software is a digital image capture and analysis tool developed by Leica. The software enables users to capture, process, and manage high-quality digital images from Leica microscopes and cameras. It provides core functionalities for image acquisition, annotation, and basic analysis.
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Lab products found in correlation
2 protocols using spot imaging software
1
Fluorescent Imaging of HECs on Patterned Substrates
2
Histochemical Analysis of Tissue Decalcification
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Explants were decalcified in 0.5 M EDTA/PBS solution for 14 changes (of 24 h) prior to dehydration, embedding in paraffin wax and sectioning at 5 µm. Slides were stained with Haematoxylin & Eosin, Safranin-O and Fast Green, Mason’s Trichrome and Picrosirius Red. Images were taken with an Olympus DP73 microscope. Polarized light microscopy to visualize collagen fibers after Picrosirius Red staining was performed with a Leica DMR microscope and SPOT imaging software.
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