BPI-9016M, which is a selective small-molecular c-Met inhibitor, was kindly provided by Better Pharmaceuticals (Zhejiang, China). BPI-9016M was dissolved in 0.5% carboxymethylcellulose sodium (CMC) for PDX treatment. For other experiments, the drug was dissolved in dimethyl sulfoxide at a stock concentration of 50 mM and stored at -80 °C. The DKK1 siRNA, miR103 mimic, miR203 mimic, miR103 inhibitor and miR203 inhibitor were constructed by RiboBio (Guangzhou, China) and the pENTER-DKK1 plasmid was purchased from Vigenebio (Maryland, USA). The transfections were performed according to the protocol provided by the manufacturer (Lipo3000, Thermo Fisher, MA, USA).
Mir 203 inhibitor
Manufactured by RiboBio
Sourced in China
The MiR-203 inhibitor is a laboratory product designed to inhibit the expression of the microRNA miR-203. This microRNA plays a role in various cellular processes. The inhibitor can be used in research applications to study the functional effects of miR-203 modulation.
Automatically generated - may contain errors
Lab products found in correlation
Mir 203 mimic, by RiboBio (1 mentions)
Mir103 inhibitor, by RiboBio (1 mentions)
Lipo3000, by Thermo Fisher Scientific (1 mentions)
Adenovirus vector, by Genechem (1 mentions)
Turbofect sirna transfection reagent, by Thermo Fisher Scientific (1 mentions)
Inhibitor nc, by RiboBio (1 mentions)
Si crnde, by RiboBio (1 mentions)
Pcdna3.1 nc, by RiboBio (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
3 protocols using mir 203 inhibitor
1
c-Met Inhibitor BPI-9016M Protocol
2
Regulation of ZEB2 by miR-203 in NPC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
miR-203 inhibitor and si-ZEB2s were purchased from RiboBio Inc, Guangzhou, China. ZEB2 cDNA was chemically synthesized and then constructed into adenovirus vector (Shanghai Genechem Co., Ltd., China). Twenty-four hours before transfection, NPC cells were plated onto a 6-well plate (Nest, Biotech, China) at 30–50% confluence. They were then transfected into cells using TurboFectTM siRNA Transfection Reagent (Fermentas, Vilnius, Lithuania) according to the manufacturer's protocol. Cells were collected after 48-72 hrs for further experiments.
3
Transfection of VEGFA Plasmids and Mimics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Si-CRNDE, miR-203 mimics, negative control (NC) mimics, miR-203 inhibitor, NC inhibitor, pmirGLO-VEGFA 3′UTR WT, pmirGLO-VEGFA 3′UTR MUT, pcDNA3.1-VEGFA (p-VEG-FA), and pcDNA3.1-NC were provided by RiboBio (Guangzhou, China). According to the manufacturer's instructions, plasmids or oligonucleotides were transfected into cells along with Lipofectamine TM 2000 (Invitrogen).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!