Cells were fixed and permeabilized with Perm/Wash Buffer (Cat. # 554723, BD Biosciences). Samples were blocked with Image-iT FX signal enhancer for 30 min and incubated for 2 hours at room temperature with primary antibodies combined with reagents of Zenon Alexa Fluor 488 (green) or 555 (red) labeling kit (Invitrogen). To detect Ki-67 in PCa cells co-cultured with MC3T3-E1 cells in vitro, human Ki-67 (cat. # ab15580, Abcam) and HLA-ABC (Cat. # 311402, BioLegend) antibodies were used as primary antibodies. To detect Ki-67 in PCa cells in bone marrow sections, human Ki-67 (cat. # ab15580, Abcam) and pan cytokeratin (cat. # ab867364, Abcam) antibodies were used as the primary antibody. To detect human TGFBR2 and TGFBR3, human TGFBR2 (cat. # ab61213, Abcam) and human TGFBR3 (cat. # ab78421, Abcam) antibodies were used as the primary antibody. After washing with PBS, the slides were mounted with ProLong Gold antifade reagent with DAPI (Invitrogen). Images were taken with Nikon A-1-B confocal microscope.
Zenon alexa fluor 488 green or 555 red labeling kit
Manufactured by Thermo Fisher Scientific
The Zenon Alexa Fluor 488 or 555 labeling kit is a reagent system designed for the rapid and efficient labeling of antibodies or other proteins with the Alexa Fluor 488 (green) or 555 (red) fluorescent dye. The kit provides a simple and convenient method for the direct conjugation of the dye to the target protein.
Automatically generated - may contain errors
Lab products found in correlation
Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (4 mentions)
Fv500 confocal microscope, by Olympus (3 mentions)
Perm wash buffer, by BD (3 mentions)
Ab61213, by Abcam (1 mentions)
Ab15580, by Abcam (1 mentions)
Hla abc, by BioLegend (1 mentions)
Cd133, by Novus Biologicals (1 mentions)
4 protocols using zenon alexa fluor 488 green or 555 red labeling kit
1
Immunofluorescence Analysis of PCa Cells
2
Immunostaining of Cells, Tumor, and Bone
3
Immunostaining of Cell Lines
4
Immunostaining of Tumor Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells, tumor sections and long bone sections were used for immunostaining. Cells were fixed and permeabilized with Perm/Wash Buffer (cat. 554723, BD Biosciences). Tumor sections were blocked with Image-iT FX signal enhancer for 30 min and incubated for 2 hours at room temperature with primary antibodies combined with reagents of Zenon Alexa Fluor 488 (green) or 555 (red) labeling kit (Invitrogen). Human GAS6 (cat. AF885, R & D System), Mer (cat. ab110108, Abcam, Burlingame, CA), p-Mer (cat. NB300-690, Novus Biologicals, Littleton, CO), CD133 (cat. 130–090–423, Miltenyi Biotec), CD44 (cat. ab6124, Abcam), CK18 (cat. ab7797, Abcam) antibodies were used as primary antibody. After washing with PBS, the slides were mounted with ProLong Gold antifade reagent with DAPI (Invitrogen). Images were taken with Olympus FV-500 confocal microscope. Human prostate tissue microarrays (TMAs) were purchased from US Biomax, Inc. (Rockville, MD). Tumors were graded using stage progressing system. Anti-human GAS6 and CK18 antibodies were applied for coimmunostaining. Staining intensity was ranked on a scale from 1 to 4 (1, negative; 2, weak; 3, moderate; and 4, strong intensity staining).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!