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Rabbit anti sox2 antibody

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The Rabbit anti-Sox2 antibody is a laboratory reagent used to detect the presence and localization of the Sox2 protein in biological samples. Sox2 is a transcription factor that plays a crucial role in the maintenance of stem cell pluripotency and the regulation of gene expression during embryonic development. This antibody can be utilized in various biochemical and immunohistochemical techniques to study the expression and distribution of Sox2 in cells and tissues.

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Lab products found in correlation

Mouse anti brdu antibody, by BD (1 mentions) Mouse anti β catenin antibody, by BD (1 mentions) Mouse monoclonal anti cd133 w6b3c1 clone, by Miltenyi Biotec (1 mentions) Mouse anti nestin antibody, by Merck Group (1 mentions) Rabbit anti olig2, by Abcam (1 mentions) Rabbit anti ki67, by Thermo Fisher Scientific (1 mentions) Triton, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Ix71 microscope, by Olympus (1 mentions) Dp2 bsw software, by Olympus (1 mentions) 710 laser scanning confocal microscope, by Zeiss (1 mentions) Rabbit anti neun antibody, by Thermo Fisher Scientific (1 mentions)

3 protocols using rabbit anti sox2 antibody

1

Antibody Profiling of Neural Stem Cells

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The antibodies used were as follows: mouse anti-β-catenin antibody, mouse anti-Stat3 antibody and mouse anti-BrdU antibody were from BD Biosciences; mouse anti-Bmi-1 antibody and rabbit anti-OLIG2 and rabbit anti-POU3F2 was from Abcam; mouse anti-PTEN antibody and rabbit anti-CHOP antibody were from Cell Signaling Technology; mouse anti-Nestin antibody and rabbit anti-Sox2 antibody were from Millipore, mouse monoclonal anti-CD133 (W6B3C1 clone) was from Miltenyi Biotec.
Xing Y., Ge Y., Liu C., Zhang X., Jiang J, & Wei Y. (2016). ER stress inducer tunicamycin suppresses the self-renewal of glioma-initiating cell partly through inhibiting Sox2 translation. Oncotarget, 7(24), 36395-36406.
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2

Immunostaining of Lens Capsule Cells

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The anterior lens capsules were fixed in 4% paraformaldehyde (in PBS) for 15 minutes immediately after being collected from surgery. After permeabilization with 0.4% Triton (Sigma–Aldrich) in PBS, the samples were incubated with the primary antibodies: rabbit anti-sox2 antibody (1/250) (Millipore, CA), mouse anti-BCRP antibody (Abcg2) (1/100) (Millipore, CA), and rabbit anti-ki67 (1/100) antibody (Thermo Scientific, IL) at room temperature overnight. Before labeling the nuclei with 4′,6-diamidino-2-phenylindole (DAPI) (0.5 μg/mL; Sigma–Aldrich), the cells were incubated for 1.5 hours with AlexaFluor 555/488-labeled secondary antibody (1/1000; Invitrogen, OR). Finally, the lens capsules were mounted on Vectashield (Vector Laboratories, CA) using carrier slices. Images were captured using an Olympus IX71 microscope (Olympus) equipped with DP2-BSW software (Olympus) and prepared with Image J software and Microsoft PowerPoint (2007).
Fu Q., Qin Z., Yu J., Yu Y., Tang Q., Lyu D., Zhang L., Chen Z, & Yao K. (2016). Effects of senescent lens epithelial cells on the severity of age-related cortical cataract in humans: A case–control study. Medicine, 95(25), e3869.
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3

Neural Progenitor and Neuron Analysis

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One brain section of ACN-Ctr and ACN-KO mice at P7 or at 1 mo of age was selected for the analysis of tdTomato reporter expression in neural progenitor cells and mature neurons, respectively. The following primary antibodies were used: rabbit anti-SOX2 antibody (1:800; Millipore), rabbit anti-NeuN antibody (1:200, Thermo Fisher). Immunolabeling was performed as previously described (Meneghini et al., 2010) ; then, sections were scanned with a Zeiss 710 confocal laser scanning microscope. Sox2 and NeuN positive cells were analyzed in their entire z-axis to assess the co-expression with the tdTomato reporter.
Tapella L., Soda T., Mapelli L., Bortolotto V., Bondi H., Ruffinatti F.A., Dematteis G., Stevano A., Dionisi M., Ummarino S., Di Ruscio A., Distasi C., Grilli M., Genazzani A.A., D'Angelo E., Moccia F, & Lim D. (2020). Deletion of calcineurin from GFAP-expressing astrocytes impairs excitability of cerebellar and hippocampal neurons through astroglial Na+ /K+ ATPase. Glia, 68(3).
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