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3 protocols using 2 6 dichlorophenolindophenol 2 6 dcpip

1

Spectrophotometric Determination of Vitamin C

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L-ascorbic acid (VC, purity > 99%), 2,6-dichlorophenolindophenol (2,6-DCPIP, purity > 98%), and 4,7-diphenyl-1,10-phenanthroline (bathophenanthroline, BP, purity > 98%) were all purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Methanol (HPLC grade) was obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). The other reagents were all obtained from Tianjin Kemiou Chemical Reagent Co. (Tianjin, China). All solutions were prepared using ultrapure water (18.25 MΩ cm) obtained using the ULUPURE water purification system (Chengdu, China).
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2

Microfluidic Droplet Merging Protocol

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The chips were filled 3 times with Novec Surface Modifer (3M), a fluoropolymer coating agent, for 30 min at 110°C on a hot plate. All experiments were conducted using the FC40 fluorinated oil (3M) implemented with a biocompatible FluoroSurfactant (Ran Biotechnologies) at different concentrations. The solutions were loaded in glass (SGE) or plastic (Terumo) syringes, that were actuated with programmable and computer controlled syringe pumps (neMESYS, Cetoni). The syringes were directly connected to the PDMS chips with PTFE tubing (Adtech). For the merging of droplet pairs, the trapping chambers were perfused with a 20% (v/v) 1H,1H,2H,2H-perfluoro-1-octanol (Sigma-Aldrich) solution dissolved in Novec™-7500 Engineered Fluid (3M) at the flowrate indicated in Table S1. The uncolored and dark droplets seen in Figure 2 are respectively made of pure water and of a 6 mM 2,6-dichlorophenolindophenol (2,6-DCPIP, Sigma-Aldrich) aqueous solution.
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3

Synthesis and Characterization of Chitosan-PVA Hydrogel

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All chemicals were of analytical grade and used without further purification. d(+)Glucose, sorbitol, yeast extract (for use in microbial growth medium), agar powder bacteriological, chitosan (low molecular weight), poly(vinyl alcohol) (molecular weight, 1 × 105–1.1 × 105 AWU), N-vinylpyrrolidone, nitric acid, potassium hexacyanoferrate (III) (K3[Fe(CN)6]), 2,6-dichlorophenolindophenol (2,6-DCPIP), sodium hydrophosphate, sodium dihydrogen phosphate, and sodium dithionite were purchased from Sigma–Aldrich Chemicals (Steinheim, Germany). All aqueous solutions were prepared using water purified and deionized (18 MΩ) with an Aqualab AL-1 Double system (Aqualab Ltd., Moscow, Russia). PVA was modified with N-vinylpyrrolidone according to [20 (link)]. Multi-walled carbon nanotubes were kindly provided by Nanotech Center Ltd. (Tambov, Russia).
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