The common procedure for western blot analysis was described in a previous publication.18 (link) The density cof the immunoreactive bands was analyzed using ImageJ software (NIH, Bethesda, MD). In the western analysis, β-actin was used as a loading control. Primary antibodies used in this study are as follows: CaMKII (santa Cruze Biotech, SC-9035), p-CaMKII (T287, Thermo Fisher Scientific, PA5-37833), RyR2 (Millipore, AB9080), p-RyR2 (S2808, Abcam, ab59225), p-RyR2 (S2814, badrilla, A010-31), PLB (Cell signaling Technology, 8495), p-PLB (Thr17, Badrilla, A010-13), SERCA2 (Abcam, ab150435), Kv4.2 (Sigma, SAB5200070), Kv4.3 (Sigma, SAB5200076), Cav1.2 (Thermo Fisher Scientific, PA5-23015), CX43 (Abcam, ab11370), and β-actin (Boster, BM0627).
P ryr2
Manufactured by Abcam
P-RyR2 is a laboratory product that specifically detects and measures the phosphorylation of ryanodine receptor 2 (RyR2), a calcium release channel found in the sarcoplasmic reticulum of cardiac muscle cells. This product can be used to study the regulation and function of RyR2 phosphorylation, which is important for cardiac muscle contraction and relaxation.
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Lab products found in correlation
Bm0627, by Boster Bio (1 mentions)
Ab9080, by Abcam (1 mentions)
Passive lysis buffer (plb), by Cell Signaling Technology (1 mentions)
P camkii, by Thermo Fisher Scientific (1 mentions)
Pka c α, by Cell Signaling Technology (1 mentions)
Pampkt172, by Cell Signaling Technology (1 mentions)
Epac1, by Cell Signaling Technology (1 mentions)
Pgc 1α antibody, by Santa Cruz Biotechnology (1 mentions)
P acc, by Cell Signaling Technology (1 mentions)
Total acc, by Cell Signaling Technology (1 mentions)
2 protocols using p ryr2
1
Western Blot Analysis of Cardiac Proteins
2
Immunoblotting Analysis of Metabolic Signaling
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Cells were lysed in RIPA buffer and subjected to immunoblotting. For tissue extraction, samples were pulverized in liquid nitrogen and homogenized in the lysis buffer. The following antibodies were used: AMPK (Cell Signaling Technology), p-AMPK (T172) (Cell Signaling Technology), p-ACC, which recognizes phosphorylated Ser 79 in ACC1 (Cell Signaling Technology), total ACC (Cell Signaling Technology), Rap1 (Cell Signaling Technology), EPAC1 (Cell Signaling Technology), PKAcα (Cell Signaling Technology), RyR2 (Abcam), p-RyR2 (Abcam), Sirt1 (Upstate Biotechology), V5 (Invitrogen) and Actin (Santa Cruz). PGC-1α acetylation was visualized by immunoprecipitation from the cell extract (500 μg) using PGC-1α antibody (Santa Cruz) followed by immunoblotting with antibody specific for acetylated lysine (Cell Signaling Technology) or for PGC-1α.
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