Gc ms 5977a

Manufactured by Agilent Technologies

Sourced in United States

The GC-MS 5977A is a gas chromatograph-mass spectrometer system designed for high-performance chemical analysis. It combines the separation capabilities of gas chromatography with the identification and quantification capabilities of mass spectrometry. The system is capable of analyzing a wide range of volatile and semi-volatile organic compounds.

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Lab products found in correlation

Hp 5ms column, by Agilent Technologies (2 mentions) No 1 filter paper, by Cytiva (1 mentions) Hp 5ms ui capillary column, by Agilent Technologies (1 mentions) Hp 5ms ultra inert column, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Agilent 7890B/5977A GC-MS system 7890B-5977A GC-MS system GC-MS 5977A MSD-7890A Agilent 7890-5977A GC-MS system 7890B-5977A GC/MSD GC-MS 7890B GC/5977A MS detector Agilent 7890B/5977A GC–MS GC-MS 7890B-5977A MS 5977A GC-MS

7 protocols using gc ms 5977a

Bioactive Metabolite Extraction from Endophytic A. terreus

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Endophytic A. terreus was grown for 14 days on potato dextrose broth (PDB) at 28 ± 2 °C under static circumstances. Afterward, the culture was filtered through Whatman No. 1 filter paper. The resulting culture filtrate was mixed with ethyl acetate in a 1:1 ratio and collected in the uppermost portion of the organic layer. Furthermore, the extraction method was repeated three to four times, pooled, and condensed at 45 °C using a rotary evaporator. After that, the ethyl acetate extract (EAE) was collected and stored at room temperature [21 (link)]. To determine the metabolic components, the EAE of A. terreus was put into a GC-MS. The GC-MS analysis was carried out using an Agilent Technologies GC-MS 5977 A at 70 eV using a computer mass spectral library (NIST, 2011 edition). The unknown components’ spectra matched the data in the GC-MS library.

Abdelaziz A.M., El-Wakil D.A., Hashem A.H., Al-Askar A.A., AbdElgawad H, & Attia M.S. (2023). Efficient Role of Endophytic Aspergillus terreus in Biocontrol of Rhizoctonia solani Causing Damping-off Disease of Phaseolus vulgaris and Vicia faba. Microorganisms, 11(6), 1487.

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Metabolic Profiling of Alternaria Crude Extract

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EA crude extract of A. alternaria was injected to GC–MS to identify the metabolic compounds. GC–MS analysis was achieved using Agilent Technologies GC–MS 5977A operating at 70 eV and computer mass spectral library (NIST, 2011 version). The spectrum of the unknown constituents was matching with the available data stored in GC–MS libraries.

Elghaffar R.Y., Amin B.H., Hashem A.H, & Sehim A.E. (2022). Promising Endophytic Alternaria alternata from Leaves of Ziziphus spina-christi: Phytochemical Analyses, Antimicrobial and Antioxidant Activities. Applied Biochemistry and Biotechnology, 194(9), 3984-4001.

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Quantification of Short-Chain Fatty Acids

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SCFAs were derivatized to their corresponding butyl esters (SCFABE) followed by GC-MS analyses using a GC-MS 5977A and HP-5MS UI capillary column from the same manufacturer (Agilent, Wilmington, DE, USA). The data were expressed in mg/gm of faecal sample.

Upadhyaya B., McCormack L., Fardin-Kia A.R., Juenemann R., Nichenametla S., Clapper J., Specker B, & Dey M. (2016). Impact of dietary resistant starch type 4 on human gut microbiota and immunometabolic functions. Scientific Reports, 6, 28797.

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Peppermint Essential Oil Extraction and Analysis

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The peppermint EO was extracted by the water distillation method using a Clevenger. For this purpose, 40 g of the aerial parts of peppermint were poured into the Clevenger and were added with 300 mL of distilled water and the extraction was performed at water boiling temperature for 3 h. After EO extraction, the required amount of sodium sulfate was added in samples and kept in a refrigerator (4 °C) in darkness for chemical analysis. The EO content and EO yield were calculated based on the following equations [6 (link)]:

Moreover, the EO constituents were analyzed using GC–MS (GC–MS; 5977A, Agilent; Stevens Creek Blvd. Santa Clara, CA, USA) and GC–FID (Agilent 7990B; Stevens Creek Blvd. Santa Clara, CA, USA), following the previously method of Amani Machiani et al. [7 (link)].

Javanmard A., Amani Machiani M., Haghaninia M., Pistelli L, & Najar B. (2022). Effects of Green Manures (in the Form of Monoculture and Intercropping), Biofertilizer and Organic Manure on the Productivity and Phytochemical Properties of Peppermint (Mentha piperita L.). Plants, 11(21), 2941.

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Analysis of C. inophyllum Nut Oil Composition

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Analysis of the chemical composition of the nut oils extracted from C. inophyllum seeds was performed using gas chromatography-mass spectrometry (GC-MS 5977A, Agilent Technologies, MSD, USA) with an HP-5ms ultra inert column (length, 30 m; internal diameter, 0.25 mm; and film thickness, 0.25 mm) at a flow rate of 1 mL/min. A sample volume of 1 ml was injected (a split ratio of 50:1) and carried out by helium gas (99.99%) at a flow rate of 2 ml/min. The injector temperature was maintained at 290 °C. The ion-source temperature was 270 °C. The oven temperature was programmed by starting at 60 °C (isothermal for 2 min), with an increase of 4 °C/min to 270 °C, then increasing at the rate of 10 °C/min to 290 °C, and ending with a 10 min isothermal at 300 °C. For mass spectra, it was done at 70 eV and fragments from 35 to 500 Da were showed. The solvent delay was 3 min, and the total GC/MS running time was 650 min. The peak areas were represented by the percentage amount of each compound and their retention time was compared to the calibration curves of the internal standards (Tab. 1) for the compound identification.

Saechan C., Kaewsrichan J., Leelakanok N., & Petchsomrit A. (2021). Antioxidant in cosmeceutical products containing Calophyllum inophyllum oil. OCL, 28, 28-28.

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Enzymatic Synthesis of Pentalenolactone Derivatives

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Preparative-scale incubations were carried out using wild-type PntM (3.8 μM), or with mutants F232A (4.4 μM), F232G (15.2 μM), F232L (5.5 μM), M81A (12.4 μM), M81C (7.2 μM), M81MBE (12 μM) or M77S (10.3 μM) in 20 mM Tris-HCl buffer (pH 8.0) containing 2.5% glycerol, 20 μg /mL spinach ferredoxin, 0.05 U/mL spinach ferredoxin-NADP reductase, pentalenolactone F (14.4 - 144 μM and farnesol (1 μM) as internal standard in a total volume of 4.00 mL. The reactions were initiated by adding 1 mM NADPH, then incubated for 4 h at 30 °C to maximize substrate consumption. The reaction was quenched with 16 μL of 2 N HCl to a final pH ∼2. The products were extracted 2× with 4 mL dichloromethane and the combined organic extracts were dried over Na₂SO₄. The concentrated extract was dissolved in 200 μL methanol and treated with trimethylsilydiazomethane (4 μL, TMS-CHN₂, 2 M solution in hexane) to generate the corresponding methyl esters. GC-MS analysis using an Agilent 5977A GC-MS and Agilent HP-5MS column and temperature program from 40 - 240 °C with an increase of 5 °C per min showed the same product profile as that for oxidation of 2 by wild-type PntM, with the exclusive product being 1-Me. No additional peaks with [M⁺]⁺m/z 308 or [M-H₂O]⁺m/z 290 corresponding to any hydroxylated derivative of 2-Me were detected.

Duan L., Jogl G, & Cane D.E. (2016). The Cytochrome P450-Catalyzed Oxidative Rearrangement in the Final Step of Pentalenolactone Biosynthesis: Substrate Structure Determines Mechanism. Journal of the American Chemical Society, 138(38), 12678-12689.

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Quantification of 1-Methyl by GC-MS

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A reference series of increasing concentrations of 1-Me (4.8, 9.7, 14.5, 19.4, and 48.4 μM) was mixed with 10 μM farnesol as internal standard and analyzed by GC-MS using an Agilent 5977A GC-MS and Agilent HP-5MS column with a temperature program from 40 °C - 240 °C with an increase of 5 °C per min. For data collection, farnesol was quantitated by Selected Ion Monitoring (SIM), up to 35 min using SIM Group A, (consisting of mass fragments m/z 41.10, 55.05, 61.00, 69.05, 81.00, 93.00, 107.00). 1-Me was quantitated after 35 min using SIM Group B (consisting of mass fragments m/z 77.00, 91.00, 105.00, 115.00, 127.00, 128.00, 129.00, 130.00, 131.00, 143.00, 145.00, 157.00, 171.00, 202.00, 290.00). The SIM peak areas for the farnesol internal standard and 1-Me were individually summed and then used to calculate the calibration curve of normalized peak area versus concentration for 1-Me (Figure S7).

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