Eclipse i5 light microscope

Left ventricle was also evaluated for histopathological analysis. Sections were
stained with hematoxylin-eosin and Masson’s trichrome methods. We used
hematoxylin-eosin stain for routine detection of pathological alterations
including necrosis, congestion, and infiltration. Masson’s trichrome method was
chosen for determining fibrosis on myocardium if any. Sections were examined and
scored by an observer who was blind to the identification of the groups using a
Nikon Eclipse i5 light microscope with a Nikon DS-Fi1c camera, and Nikon NIS
Elements version 4.0 image analysis systems (Nikon Instruments Inc., Tokyo,
Japan). Myocardial damage was scored in terms of necrosis, congestion, and
mononuclear cell infiltration. Each data was scored as: 0: absent, 1: minimal,
2: moderate, 3: severe damage.

For histopathological analysis, ovarian tissues were fixed in 10% neutral buffered formaldehyde and embedded in paraffin. Cross-sections (5 μm) were obtained and stained with haematoxylin&eosin (H&E). Ovarian damage was evaluated by considering stromal congestion, oedema and infiltration, follicular degeneration and oedema and necrosis in the corpus luteum. The severity of these parameters was calculated as 0: absent, 1: minimal, 2: moderate and 3: severe (17 (link)). Sections were examined under light microscopy (Nikon Eclipse i5 light microscope with a Nikon DS-Fi1c camera and the Nikon NIS Elements version 4.0 image analysis systems. Nikon Instruments Inc., Tokyo, Japan). All tissue samples were evaluated by a single histologist who was blinded to the groups.