Donkey anti goat af555

Manufactured by Thermo Fisher Scientific

Donkey anti-goat–AF555 is a secondary antibody conjugated with the fluorescent dye Alexa Fluor 555. It is used to detect and visualize goat primary antibodies in various immunoassay applications, such as immunofluorescence, flow cytometry, and Western blotting.

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Lab products found in correlation

Lsm 700 confocal microscope, by Zeiss (2 mentions) Goat anti rat af488, by Thermo Fisher Scientific (1 mentions) Dextran af488, by Thermo Fisher Scientific (1 mentions) Wga af647, by Thermo Fisher Scientific (1 mentions) Chicken anti rabbit af488, by Thermo Fisher Scientific (1 mentions) Dq green bsa, by Thermo Fisher Scientific (1 mentions) Lamp1, by BD (1 mentions) Chicken anti mouse af488, by Thermo Fisher Scientific (1 mentions) Donkey serum, by Jackson ImmunoResearch (1 mentions) Rabbit anti human cd3, by Agilent Technologies (1 mentions) Goat anti human il17, by R&D Systems (1 mentions) Fc blocker, by Miltenyi Biotec (1 mentions) Donkey anti rabbit af488, by Thermo Fisher Scientific (1 mentions) Target retrieval solution, by Agilent Technologies (1 mentions)

3 protocols using donkey anti goat af555

Fluorescent Markers for Organelle Tracking

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Primary antibodies included the following: EEA1 (Santa Cruz Biotechnology, sc-6415), Rab7 (Abcam, ab 137029), Lamp1 (BD Pharmingen, 1D4B), GFP (Life Technologies, A11122). Secondary antibodies included the following: donkey anti-goat–AF555 (Invitrogen, A21432), goat anti-rat–AF488 (Invitrogen A11006), chicken anti-rabbit–AF488 (Invitrogen, A21441).
Other reagents included the following: AF594/Fab (Jackson ImmunoResearch Laboratories Inc., 309-586-003), A488 phalloidin (Life Technologies, A12379), AF555 phalloidin (Life Technologies, A34055), DQ Green BSA (Life Technologies, D12050), Tf-AF647 (Life Technologies, T23366), dextran-AF488 (Life Technologies, ID7178), WGA-AF647 (Life Technologies, W32466).

Piperno G.M., Naseem A., Silvestrelli G., Amadio R., Caronni N., Cervantes-Luevano K.E., Liv N., Klumperman J., Colliva A., Ali H., Graziano F., Benaroch P., Haecker H., Hanna R.N, & Benvenuti F. (2020). Wiskott-Aldrich syndrome protein restricts cGAS/STING activation by dsDNA immune complexes. JCI Insight, 5(17), e132857.

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Fibrinogen-Based Fluorescent Assay

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Human plasma fibrinogen containing > 90% clottable proteins was obtained from Calbiochem (San Diego, CA) and bovine thrombin was purchased from Siemens Healthcare (Plainfield, IN). The 96-well microtiter plates (clear, polystyrene) used for fluorescence measurements were obtained from Corning Incorporated (Corning, NY). S-nitroso-acetyl penicillamine (SNAP), N-acetyl penicillamine (NAP), S-nitroso-glutathione (GSNO), glutathione (GSH) and sodium nitroprusside (SNP) were purchased from Sigma Aldrich (Milwaukee, WI). NO gas at 100 or 1000 ppm (balance nitrogen gas) was purchased from Praxair (Detroit, MI). The mouse anti-human fibrinogen alpha, rabbit anti-human fibrinogen beta and goat anti-human fibrinogen gamma antibodies were obtained from Santa Cruz (Dallas, TX). The secondary antibodies chicken anti-mouse-AF488, donkey anti-goat-AF555 and chicken anti-rabbit-AF647 were from Invitrogen (Life Tech. Eugene, OR). All other reagents were of the highest purity available.

Major T.C., Brisbois E.J., Meyerhoff M.E, & Bartlett R.H. (2018). Attenuation of Thrombin-Mediated Fibrin Formation via Changes in Fibrinogen Conformation Induced by Reaction with S-nitroso-N-acetylpenicillamine, but not S-nitrosoglutathione. Journal of materials chemistry. B, 6(47), 7954-7965.

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Multicolor Immunofluorescence of FFPE Tissues

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Archived formalin fixed and paraffin embedded (FFPE) tissues were identified by searching the Veterinary Medical & Administrative Computer System at UCD School of Veterinary Medicine. Five μm tissue sections were cut and mounted onto glass slides. Tissues were deparaffinized with xylene and a serial ethanol dilution. Slides were boiled at 95°C for 20 minutes (Target Retrieval Solution, DAKO, Carpinteria, CA) following blocking with 15% donkey serum (Jackson ImmunoResearch Laboratories Inc, West Grove, PA) and 1% Fc blocker (Miltenyi Biotec) for 45 minutes. Slides were than incubated with rabbit anti-human CD3 (DAKO) and goat anti-human IL17 (R&D) antibodies at 4°C overnight. Slides were then washed and stained with donkey anti-rabbit AF488 (Invitrogen) and donkey anti-goat AF555 (Invitrogen) for 1 hour at room temperature in the dark. Finally, slides were mounted with DAPI containing mounting media (Vector Laboratories, Burlingame, CA). Images were acquired with a LSM 700 confocal microscope (Zeiss, Pleasanton, CA).

Kol A., Walker N.J., Nordstrom M, & Borjesson D.L. (2016). Th17 Pathway As a Target for Multipotent Stromal Cell Therapy in Dogs: Implications for Translational Research. PLoS ONE, 11(2), e0148568.

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