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Pax6 pe

Manufactured by BD
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PAX6 PE is a laboratory equipment product designed for research purposes. It is used to detect and quantify the expression of the PAX6 gene, which plays a crucial role in the development and function of various tissues, including the eye, brain, and pancreas. The core function of this product is to enable researchers to measure and analyze PAX6 gene expression levels in biological samples.

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Lab products found in correlation

Sox2 percp cy5, by BD (2 mentions) Satb2 alexa fluor 647, by Abcam (2 mentions) Rnasin plus rnase inhibitor, by Promega (2 mentions) Fcr blocking reagent, by Miltenyi Biotec (2 mentions) Eomes pe cy7, by Thermo Fisher Scientific (2 mentions) Facsaria 3, by BD (1 mentions) Cd34 pe, by BD (1 mentions) Cd45 apc, by BD (1 mentions) Cell dissociation buffer, by Thermo Fisher Scientific (1 mentions) Cd43 fitc, by BD (1 mentions)

Spelling variants of this product

PE-conjugated anti-PAX6 (O18-1330; (2 citations)

3 protocols using pax6 pe

1

Multilineage Differentiation Assessment

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Single-cell suspensions were stained with fluorochrome-conjugated antibody to evaluate pluripotency and hematopoietic and neural differentiation. Briefly, cells were dissociated with cell dissociation buffer (Gibco) for 10 min, and then resuspended in phosphate-buffered saline supplemented with 3% v/v fetal bovine serum. The following antibodies were used for visualization: CD34-PE (1:100; BD Biosciences), CD43-FITC (1:100; BD Biosciences), CD45-APC (1:100; BD Biosciences), PAX6-PE (1:100; BD Biosciences), NESTIN-FITC (1:100; Millipore, Burlington, MA, USA), and LIVE/DEAD-APC-Cy7 (1: 7500; Thermo Fisher Scientific). Fluorescence activated cell sorting (FACS) analysis was performed with a BD FACSAriaTM III (BD Biosciences), and all data were analyzed with FlowJo software (Tree Star, Ashland, OR, USA).
Choi S.A., An J.H., Lee S.H., Lee G.H., Yang H.J., Jeong P.S., Cha J.J., Lee S., Park Y.H., Song B.S., Sim B.W., Kim Y.H., Kim J.S., Jin Y.B., Huh J.W., Lee S.R., Lee J.H, & Kim S.U. (2019). Comparative Evaluation of Hormones and Hormone-Like Molecule in Lineage Specification of Human Induced Pluripotent Stem Cells. International Journal of Stem Cells, 12(2), 240-250.
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2

Flow Cytometry Analysis of Pluripotency Markers

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The cell pellet was thawed on ice and resuspended in PBS containing 0.1% Triton-X-100 for 15 minutes. The cells were then washed twice with PBS and resuspended in 5% BSA in PBS for staining. Staining proceeded for at least one hour with FcR Blocking Reagent (Miltenyi Biotech, 1/20 dilution), EOMES PE-Cy7 (Invitrogen, Cat 25-4877-42, Clone WD1928, Lot 1923396, 1/10 dilution), PAX6 PE (BD Biosciences, Cat 561552, Clone O18–1330, Lot 8187686, 1/10 dilution), SOX2 PerCP-Cy5.5 (BD Biosciences, Cat 561506, Clone O38– 678, Lot 8165744, 1/10 dilution), and SATB2 Alexa Fluor 647 (Abcam, Cat ab196536, Clone EPNCIR130A, Lot GR3208103-I and GR228747–2, 1/100 dilution). After staining, the cells were centrifuged for 5 minutes at 500 g, and the pellet was diluted into PBS. When sorting cells for RNA-seq, 1% RNasin Plus RNase Inhibitor (Promega) was added to all buffers, and acetylated BSA was used to prepare 5% BSA in PBS for staining.
Song M., Pebworth M.P., Yang X., Abnousi A., Fan C., Wen J., Rosen J.D., Choudhary M.N., Cui X., Jones I.R., Bergenholtz S., Eze U.C., Juric I., Li B., Maliskova L., Lee J., Liu W., Pollen A.A., Li Y., Wang T., Hu M., Kriegstein A.R, & Shen Y. (2020). Cell type-specific 3D epigenomes in the developing human cortex. Nature, 587(7835), 644-649.
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3

Flow Cytometry Analysis of Pluripotency Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cell pellet was thawed on ice and resuspended in PBS containing 0.1% Triton-X-100 for 15 minutes. The cells were then washed twice with PBS and resuspended in 5% BSA in PBS for staining. Staining proceeded for at least one hour with FcR Blocking Reagent (Miltenyi Biotech, 1/20 dilution), EOMES PE-Cy7 (Invitrogen, Cat 25-4877-42, Clone WD1928, Lot 1923396, 1/10 dilution), PAX6 PE (BD Biosciences, Cat 561552, Clone O18–1330, Lot 8187686, 1/10 dilution), SOX2 PerCP-Cy5.5 (BD Biosciences, Cat 561506, Clone O38– 678, Lot 8165744, 1/10 dilution), and SATB2 Alexa Fluor 647 (Abcam, Cat ab196536, Clone EPNCIR130A, Lot GR3208103-I and GR228747–2, 1/100 dilution). After staining, the cells were centrifuged for 5 minutes at 500 g, and the pellet was diluted into PBS. When sorting cells for RNA-seq, 1% RNasin Plus RNase Inhibitor (Promega) was added to all buffers, and acetylated BSA was used to prepare 5% BSA in PBS for staining.
Song M., Pebworth M.P., Yang X., Abnousi A., Fan C., Wen J., Rosen J.D., Choudhary M.N., Cui X., Jones I.R., Bergenholtz S., Eze U.C., Juric I., Li B., Maliskova L., Lee J., Liu W., Pollen A.A., Li Y., Wang T., Hu M., Kriegstein A.R, & Shen Y. (2020). Cell type-specific 3D epigenomes in the developing human cortex. Nature, 587(7835), 644-649.
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