1200 series refractive index detector

Manufactured by Wyatt Technology

The 1200 Series refractive index Detector is a laboratory instrument designed to measure the refractive index of a sample. It provides accurate and reliable measurements of the refractive index, which is a fundamental property of materials and is widely used in various scientific and industrial applications.

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Lab products found in correlation

Astra 6, by Wyatt Technology (2 mentions) Dawn helios 8 mals unit, by Wyatt Technology (2 mentions) Akta purifier fplc system, by GE Healthcare (1 mentions) Kta purifier fplc system, by GE Healthcare (1 mentions) Superdex 200 increase 10 300 gl column, by GE Healthcare (1 mentions)

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Refractive index detector (14 citations)

2 protocols using 1200 series refractive index detector

SEC-MALS Analysis of RBD-RPA70N Complex

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SEC-MALS was performed on an AKTA Purifier FPLC system (GE) connected to an Agilent Technologies 1200 Series refractive index Detector and a Wyatt Technologies Dawn Helios 8+ MALS unit. A Superdex 75 increase 10/300 GL (24 ml) column was equilibrated in running buffer consisting of 20 mM HEPES (pH 7.1), 80 mM NaCl, 0.5 mM TCEP. The flow was maintained at a consistent 0.5 ml min⁻¹ and sample injections of 100 μl from a static loop were initiated at the 0 ml point of each run. Ultraviolet, refractive index, light scattering (LS) and Quasi-Elastic LS values were recorded using ASTRA 6.1 (Wyatt) software. Data were collected using samples of RBD at 185 μM with RPA70N E7R added at 0, 1, 2 and 4 × molar ratios. Estimated molecular weights for RBD and its saturated complexes were calculated using the Zimm algorithm surrounding the peak maximum.

Guilliam T.A., Brissett N.C., Ehlinger A., Keen B.A., Kolesar P., Taylor E.M., Bailey L.J., Lindsay H.D., Chazin W.J, & Doherty A.J. (2017). Molecular basis for PrimPol recruitment to replication forks by RPA. Nature Communications, 8, 15222.

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Molecular Mass Determination via SEC-MALLS

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Size-exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLS) was performed on an ÄKTA Purifier FPLC system (GE Healthcare) in-line with an Agilent Technologies 1200 Series refractive index Detector and a Dawn Helios 8+ MALS unit (Wyatt). 100 μL of sample at 15 μM was injected from a static loop beginning at the 0 ml point of each run onto a Superdex 200 increase 10/300 GL column (GE Healthcare) at a flow rate of 0.5 ml/min. Samples were assayed at 25°C in 20 mM Tris-HCl (pH 8.5), 2mM DTT, and 8 mM NaN₃ at two different salt concentrations (50 mM NaCl and 500 mM NaCl). Refractive index and light scattering data were recorded and analyzed using ASTRA 6.1 (Wyatt) software. Molecular masses were calculated across distinct eluted peaks using the Zimm algorithm with a dn/dc value of 0.185 ml/g.

Kendall A.K., Xie B., Xu P., Wang J., Burcham R., Frazier M.N., Binshtein E., Wei H., Graham T.R., Nakagawa T, & Jackson L.P. (2020). Mammalian Retromer Is an Adaptable Scaffold for Cargo Sorting from Endosomes. Structure (London, England : 1993), 28(4), 393-405.e4.

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