For cell cycle analysis through propidium iodide (PI) staining, 1×105 cells were seeded in 6-well plate and treated with 50 or 100 µM decursin or siRNA for 48 h. Afterwards, the cells were fixed in 70% ice-cold ethanol overnight at −20°C and incubated with FxCycle™ PI/RNase Staining Solution (cat. no. F10797; Invitrogen; Thermo Fisher Scientific, Inc.) for 30 min at room temperature. Fluorescence was analyzed using flow cytometry (Beckman Coulter) and the Kaluza analysis program (version 1.2; Beckman Coulter).
Kaluza analysis program version 1
Manufactured by Beckman Coulter
Sourced in United States
The Kaluza analysis program (version 1.2) is a software tool designed for data analysis in flow cytometry. It provides a suite of analytical capabilities to process and visualize flow cytometry data.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using kaluza analysis program version 1
1
Cell Cycle Analysis by Propidium Iodide Staining
2
Cell Cycle Analysis by Flow Cytometry
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For cell cycle analysis, 1 × 105 cells were seeded in a 6-well plate. After two washes with ice-cold PBS, cells were fixed in 70% ice-cold ethanol overnight at 4 °C. Cells were washed again with ice-cold PBS and incubated with FxCycle™ PI/RNase Staining Solution (Invitrogen) according to the manufacturer’s instructions. Fluorescence was analyzed using a flow cytometer (Beckman Coulter, Brea, CA, USA) and the Kaluza analysis program (version 1.2; Beckman Coulter).
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