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Sybr green sg

Manufactured by Thermo Fisher Scientific
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Sourced in Belgium

SYBR Green (SG) is a fluorescent dye used for the detection and quantification of DNA. It binds to double-stranded DNA and emits a fluorescent signal upon excitation, allowing for the monitoring of DNA amplification in real-time PCR applications.

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Lab products found in correlation

Propidium iodide, by Merck Group (2 mentions) Facsverse, by BD (1 mentions)

2 protocols using sybr green sg

1

Quantifying Bacterial Viability via Flow Cytometry

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Bacterial cell densities were measured using a BD FacsVerse flow cytometer and live cells were distinguished from damaged cells using SYBR Green (SG; Thermo Fisher, Merelbeke, Belgium)/propidium iodide (PI;Merck KGaA, Darmstadt, Germany) staining, as previously described [34 (link)]. Proportional values obtained with Illumina sequencing were converted into absolute quantities by correcting with live bacterial cell counts. Bacterial cells were separated from medium debris and signal noise by applying a threshold level of 200 on the SYTO channel.
Calatayud M., Börner R.A., Ghyselinck J., Verstrepen L., Medts J.D., den Abbeele P.V., Boulangé C.L., Priour S., Marzorati M, & Damak S. (2021). Water Kefir and Derived Pasteurized Beverages Modulate Gut Microbiota, Intestinal Permeability and Cytokine Production In Vitro. Nutrients, 13(11), 3897.
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2

Quantifying Bacterial Cell Densities

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Bacterial cell densities were measured using an AccuriC6 flow cytometer and intact cells were distinguished from damaged cells using SYBR Green (SG; Thermo Fisher)/propidium iodide (PI; Sigma) staining, as previously described (79 (link)). During coculture, bacterial cells were recovered from the cell layer by gently pipetting up and down the apical washing fluid (PBS with calcium and magnesium ions), and the bacterial densities in the apical washing fluid were determined by using flow cytometry.
De Rudder C., Calatayud Arroyo M., Lebeer S, & Van de Wiele T. (2020). Dual and Triple Epithelial Coculture Model Systems with Donor-Derived Microbiota and THP-1 Macrophages To Mimic Host-Microbe Interactions in the Human Sinonasal Cavities. mSphere, 5(1), e00916-19.
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