Accuri c6 cflow sampler software

Fluorescence intensity was measured with a BD Accuri C6 flow cytometer equipped with a CSampler plate adapter using excitation wavelengths of 488 and 640 nm and an emission detection filter at 533 nm (FL1 channel). A total of 10,000 events above a 400,000 FSC-H threshold (to exclude debris) were recorded for each sample with and core size of 22 mm using the Accuri C6 CFlow Sampler software. Cytometry data were exported as FCS 3.0 files and processed using the flowCore R software package and custom R scripts (Supplementary Software 1) to obtain the mean FL1-A value at each data point.

Fluorescence intensity was measured with a BD Accuri C6 flow cytometer equipped with a CSampler plate adapter at room temperature using excitation wavelengths of 488 and 640 nm and an emission detection filter at 533 nm (FL1 channel). A total of 10,000 (20,000 for the bistable switch samples) events above a 400,000 FSC-H threshold (to exclude debris) were recorded for each sample using the Accuri C6 CFlow Sampler software at fast flow rate (66 µL/min, 22 μ core). Cytometry data were exported as FCS 3.0 files and processed using a custom Python script to obtain the mean FL1-A value for each data point.