Bp 9200

Manufactured by Vector Laboratories

Sourced in United States

The BP-9200 is a high-performance benchtop power supply designed for laboratory and industrial applications. It provides a stable and adjustable direct current (DC) output with a range of voltage and current settings. The BP-9200 is suitable for powering a variety of electronic devices and components during testing, development, and research activities.

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Lab products found in correlation

Ba 3000, by Vector Laboratories (2 mentions) Z0622, by Agilent Technologies (2 mentions) Pk 6100, by Vector Laboratories (2 mentions) Ma5 14238, by Thermo Fisher Scientific (1 mentions) Mbs442004, by MyBioSource (1 mentions) Avidin biotin peroxidase complex solution abc solution, by Vector Laboratories (1 mentions) Ckx41 light microscope, by Olympus (1 mentions) Hematoxylin and eosin h e, by Merck Group (1 mentions) In situ cell death detection kit, by Roche (1 mentions) Ab48506, by Abcam (1 mentions)

Spelling variants of this product

BP-9200/9100 (2 citations)

4 protocols using bp 9200

Histopathologic Analysis of Zr-89 PET Tumor Samples

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Tissue samples harvested from PET-positive mice were fixed in 10% neutral buffered formalin. A period of 10 half-lives of Zr-89 (∼33 days total) was allowed to pass before processing the samples for histopathologic analysis. Paraffin-embedded blocks were sliced to obtain 5-μm-thick sections, and slides were stained with hematoxylin and eosin (H&E) and IHC. IHC staining of cryosections of ovarian patient tumors was done using muAR9.6 as the primary antibody at 1:100 dilution and a biotinylated goat anti-mouse (H + L) secondary antibody (BP-9200; Vector Labs) at 1:500 dilution. IHC staining of OVCAR3 tumors and lymph node sections was performed using huAR9.6 as the primary antibody at 1:100 dilution and a biotinylated goat anti-human (H + L) secondary antibody (BA-3000; Vector Labs) at 1:500 dilution. Pan-cytokeratin staining of sections from OVCAR3 tumors and lymph nodes was performed using a rabbit polyclonal antibody (Z0622; Dako) as the primary antibody and a biotinylated anti-rabbit IgG (H + L; PK-6100; Vector Labs) as the secondary antibody–both used at 1:500 dilution. Histopathologic analysis was performed in a blinded manner by a board-certified veterinary pathologist (AP).

Sharma S.K., Mack K.N., Piersigilli A., Pourat J., Edwards K.J., Keinänen O., Jiao M.S., Zhao H., White B., Brooks C.L., de Stanchina E., Madiyalakan M.R., Hollingsworth M.A., Radhakrishnan P., Lewis J.S, & Zeglis B.M. (2021). ImmunoPET of Ovarian and Pancreatic Cancer with AR9.6, a Novel MUC16-Targeted Therapeutic Antibody. Clinical Cancer Research, 28(5), 948-959.

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Immunohistochemical Analysis of Cartilage Biomarkers

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For immunohistochemistry of indicated biomarkers, deparaffinized and rehydrated sections were first subjected to sequential enzymatic digestion with 250 U/mL hydraulinase, 100 mU/mL chondroitinase ABC, and 0.1 % trypsin for 60 min at 37 °C. Sections were blocked with 5% normal goat serum for 60 min at room temperature. Tissues were incubated with anti-Aggrecan Neoeptiope (NITEGE) (1:50, MBS442004, Mybiosource) or anti-MMP13 (1:25, MA5–14238, ThermoFisher) primary antibodies. Normal mouse IgG (sc-2025, Santa Cruz Biotechnology) was used for isotype controls at concentrations matching those of primary antibodies. Sections were then washed thrice in PBS prior to 30-minute incubation with biotin-conjugated Goat Anti-Mouse IgG (H+L) (1:500, BP-9200, Vector Laboratories). Endogenous peroxidase was blocked and detection was performed using VECTASTAIN® ABC-HRP Kit with signal development carried out using 3,3-diaminobenzidine. Slides were counterstained with 0.5 % Methyl green.

Katyal P., Hettinghouse A., Meleties M., Hasan S., Chen C., Cui M., Sun G., Menon R., Lin B., Regatte R., Montclare J.K, & Liu C.J. (2022). Injectable Recombinant Block Polymer Gel for Sustained Delivery of Therapeutic Protein in Post Traumatic Osteoarthritis. Biomaterials, 281, 121370.

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Histological Analysis of Fixed Liver Samples

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The fixed livers were processed for paraffin embedding and sectioned to 5-μm thickness. Sections were stained with Hematoxylin and Eosin (H&E) (Sigma-Aldrich, St. Louis, MO, USA) by a standard protocol.
For 4-Hydroxynonenal (4-HNE) immunehistochemical staining, sections were boiled in citric acid buffer (10 mM, pH 6.0) for 20 min for antigen retrieval. After blocking in 10% normal horse serum, sections were incubated with 4-HNE antibody (ab48506, Abcam) overnight at 4 °C. Sections were then incubated with a biotinylated secondary antibody (BP-9200, Vector Laboratories, Burlingame, CA, USA) for 1 h at room temperature. Sections were washed and incubated in avidin–biotin–peroxidase complex solution (ABC solution; Vector Laboratories) and developed using a 3,3ʹ-diaminobenzidine (DAB) Peroxidase Substrate Kit (Vector Laboratories). TUNEL assay was performed using an in-situ cell death detection kit (Roche Molecular Biochemicals, Mannheim, Germany) according to the manufacturer’s protocol. All images were captured using a CKX41 light microscope (Olympus, Tokyo, Japan).

Kim S.R., Park E.J., Dusabimana T., Je J., Jeong K., Yun S.P., Kim H.J., Cho K.M., Kim H, & Park S.W. (2020). Platycodon grandiflorus Fermented Extracts Attenuate Endotoxin-Induced Acute Liver Injury in Mice. Nutrients, 12(9), 2802.

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Immunohistochemical Analysis of Tumor Samples

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Tissue samples harvested from PET-positive mice were fixed in 10% neutral buffered formalin. A period of ten half-lives of Zr-89 (~33 d total) was allowed to pass before processing the samples for histopathologic analysis. Paraffin-embedded blocks were sliced to obtain 5 μm thick sections, and slides were stained with hematoxylin and eosin (H&E) and IHC. IHC staining of cryosections of ovarian patient tumors was done using muAR9.6 as the primary antibody at 1:100 dilution and a biotinylated goat anti-mouse (H+L) secondary antibody (BP-9200; Vector labs) at 1:500 dilution. IHC staining of OVCAR3 tumors and lymph node sections was performed using huAR9.6 as the primary antibody at 1:100 dilution and a biotinylated goat anti-human (H+L) secondary antibody (BA-3000; Vector labs) at 1:500 dilution. Pan-cytokeratin staining of sections from OVCAR3 tumors and lymph nodes was performed using a rabbit polyclonal antibody (Z0622; Dako) as the primary antibody and a biotinylated anti-rabbit IgG (H+L) (PK-6100; Vector labs) as the secondary antibody – both used at 1:500 dilution. Histopathologic analysis was performed in a blinded manner by a board-certified veterinary pathologist (AP).

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