Spheroids were lysed in lysis buffer (0.5% TritonX-100, 150 mM NaCl, 20 mM Tris-HCl pH7.5). The lysates were sheared with a 21-gauge needle, incubated on ice for 30 minutes and clarified by centrifugation at 20,817 × g for 15 minutes at 4°C. The extracted proteins were separated by SDS-PAGE and transferred to immobilon transfer membrane (Millipore) for Western blotting analyses. The primary antibodies were anti-YAP1 pAb (#4912 Cell Signaling, 1:500), anti-Fibronectin pAb (F3648, Sigma Aldrich, 1:1000), anti-ARHGAP18 pAb (1:10000)17 (link),21 (link), anti-MYH9 pAb (#3403 Cell signaling, 1:1000), anti-Phospho Ser1943-MYH9 pAb (#5026 Cell Signaling, 1:1000), anti-MYH10 mAb (#8824 Cell Signaling, 1:1000), anti-Phospho-Ser19 MLC2 (#3675, Cell signaling, 1:100), and anti-GAPDH mAb (sc32233, Santa Cruz, 1:5000).
Anti arhgap18 pab
Manufactured by Cell Signaling Technology
Anti-ARHGAP18 pAb is an antibody product that specifically binds to the ARHGAP18 protein. ARHGAP18 is a GTPase-activating protein (GAP) that regulates the activity of the Rho family of small GTPases. The antibody can be used to detect and study the ARHGAP18 protein in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh mab, by Santa Cruz Biotechnology (2 mentions)
Anti yap1 pab, by Cell Signaling Technology (2 mentions)
Anti fibronectin pab, by Merck Group (2 mentions)
Anti myh9 pab, by Cell Signaling Technology (2 mentions)
Anti phospho ser1943 myh9 pab, by Cell Signaling Technology (2 mentions)
Anti myh10 mab, by Cell Signaling Technology (2 mentions)
Anti phospho mlc2 ser19, by Cell Signaling Technology (2 mentions)
Immobilon transfer membrane, by Merck Group (2 mentions)
2 protocols using anti arhgap18 pab
1
Spheroid Lysis and Protein Analysis
2
Spheroid Lysis and Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Spheroids were lysed in lysis buffer (0.5% TritonX-100, 150 mM NaCl, 20 mM Tris-HCl pH7.5). The lysates were sheared with a 21-gauge needle, incubated on ice for 30 minutes and clarified by centrifugation at 20,817 × g for 15 minutes at 4°C. The extracted proteins were separated by SDS-PAGE and transferred to immobilon transfer membrane (Millipore) for Western blotting analyses. The primary antibodies were anti-YAP1 pAb (#4912 Cell Signaling, 1:500), anti-Fibronectin pAb (F3648, Sigma Aldrich, 1:1000), anti-ARHGAP18 pAb (1:10000)17 (link),21 (link), anti-MYH9 pAb (#3403 Cell signaling, 1:1000), anti-Phospho Ser1943-MYH9 pAb (#5026 Cell Signaling, 1:1000), anti-MYH10 mAb (#8824 Cell Signaling, 1:1000), anti-Phospho-Ser19 MLC2 (#3675, Cell signaling, 1:100), and anti-GAPDH mAb (sc32233, Santa Cruz, 1:5000).
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