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Rabbit anti akt 9272

Manufactured by Cell Signaling Technology
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Rabbit anti-AKT (9272) is a primary antibody product from Cell Signaling Technology. It is designed to detect AKT protein levels in various biological samples.

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Lab products found in correlation

Rat anti cd68, by BioLegend (1 mentions) Rat anti cd45, by BD (1 mentions) Cy3 conjugated secondary antibody, by Dianova (1 mentions) Ab9377, by Abcam (1 mentions) Ab21027, by Abcam (1 mentions) Ab74073, by Abcam (1 mentions) Ab16667, by Abcam (1 mentions) Goat anti lyve1 af2125, by R&D Systems (1 mentions) Alexa fluor 647, by Dianova (1 mentions) Alexa fluor 488, by Dianova (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Rabbit anti p akt ser473, by Cell Signaling Technology (1 mentions) Rabbit anti gapdh, by Merck Group (1 mentions) Recombinant human pdgf bb, by Thermo Fisher Scientific (1 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Phosphatase inhibitor, by Merck Group (1 mentions) Ripa buffer, by Merck Group (1 mentions) Chloroform, by Merck Group (1 mentions) Pierce micro bca protein assay, by Thermo Fisher Scientific (1 mentions) Gradient polyacrylamide gel, by Bio-Rad (1 mentions)

Spelling variants of this product

Anti-AKT (1447 citations) Rabbit anti-AKT (194 citations) AKT (9272) (33 citations) Anti-Akt (#9272) (29 citations) Rabbit polyclonal anti-AKT (9272; (2 citations)

7 protocols using rabbit anti akt 9272

1

Comprehensive Immunohistochemistry Panel

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Primary antibodies: Rabbit anti-cleaved Caspase 3 (9661S, Cell Signaling Technology, Danvers, USA), rabbit anti-Ki-67 (ab16667, Abcam, Cambridge, UK), rat anti-CD31 (DM3614P, Dianova, Hamburg, Germany), goat anti-CD32b (AF1460, R&D Systems, Minneapolis, USA), goat anti-Lyve1 (AF2125, R&D Systems, Minneapolis, USA), rat anti-Endomucin (14–5851–82, Thermo Fisher Scientific, Waltham, USA), rabbit anti-TRP-2 (ab74073, Abcam, Cambridge, UK), rabbit anti-wide spectrum cytokeratin (ab9377, Abcam, Cambridge, UK), goat anti-alpha smooth muscle actin (ab21027, abcam, Cambridge, UK), rat anti-CD45 (550539, BD Biosciences, Franklin Lakes, USA), rat anti-CD68 (137002, BioLegend, San Diego, USA), rabbit anti-Melan A (NBP1-30151, Novus, Minneapolis, USA). For Western blotting: rabbit anti-Akt (9272S, Cell Signaling Technology, Danvers, USA), rabbit anti-phospho-Akt (9271S, Cell Signaling Technology, Danvers, USA). Secondary antibodies: Alexa-Fluor 488, Alexa-Fluor 647 and Cy3-conjugated secondary antibodies were purchased from Dianova (Hamburg, Germany). For Western Blotting a rabbit anti-IgG HRP conjugated antibody was used. (Merck, Darmstadt, Germany).
Dietsch B., Weller C., Sticht C., de la Torre C., Kramer M., Goerdt S., Géraud C, & Wohlfeil S.A. (2023). Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern. BMC Cancer, 23, 436.
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2

Biodegradable Polyester Urethane Copolymer Characterization

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A biodegradable polyester urethane block copolymer, commercially available as DegraPol15 (DP) (Mw = 65 kDa) was kindly provided Ab Medica, Italy. The polymer was produced according to the procedure described earlier25 ,44 . Chloroform, ≥99.8% (0.5–1.0% ethanol as stabilized), 1,1,1,3,3,3-hexafluoro-2-propanol (HFP) and L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate, ≥95%, RPMI vitamins solution, RIPA buffer, phosphatase inhibitors and 0.5 M EDTA solution were purchased from Sigma-Aldrich, Switzerland. Recombinant human PDGF-BB and PDGF-BB ELISA kit were purchased from PeproTech. Ham’s F12 cell culture media, gentamicin, amphotericin B and Fetal Bovine Serum (FBS) were bought from Biowest, while non-essential amino acids solution, Pierce micro BCA protein assay and Pierce ECL Western Blotting Substrate were purchased from Thermo Scientific. PrimariaTM tissue culture plates were purchased from Corning. Primary antibodies used for western blot included rabbit anti Akt (9272 S, Cell Signaling Technology), rabbit anti pAkt (Ser473) (4060 S, Cell Signaling Technology) and rabbit anti GAPDH (G9545, Sigma Aldrich). Donkey anti rabbit antibody conjugated with HRP (Jackson) was used as secondary antibody for western blot. 4–20% gradient polyacrylamide gels were purchased from Bio-Rad, Switzerland.
Evrova O., Kellenberger D., Scalera C., Calcagni M., Giovanoli P., Vogel V, & Buschmann J. (2019). Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds. Scientific Reports, 9, 15117.
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3

Quantifying Akt Phosphorylation in Tissues

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Liver, skeletal muscle and EPI tissue samples were lysed with RIPA buffer (89901 ThermoFisher Scientific, Waltham, MA, USA) with protease inhibitor mini tablets (A32955 ThermoFisher Scientific) and phosphatase inhibitor mini tablets (A32957 ThermoFisher Scientific). Equal amounts of protein lysates were separated via SDS-PAGE and transferred to nitrocellulose membranes by electroblotting. Subsequently, the membranes were blocked using Membrane Blocking Solution (000105, Life Technologies®) for 1 hour at room temperature, then placed in primary antibodies (rabbit anti-Akt, [9272S], rabbit anti-PhosphoAktSer473 [4060S] and rabbit anti-βactin [4970S] 1:1000 dilution all from Cell Signaling Technology® 9272S, Danvers, MA, USA) incubation in Membrane Blocking Solution, overnight at 4 °C. After washing the membranes 3 × 10 min in PBS w/0.1% Tween-20, they were incubated in darkness at room temperature for 1 hour with fluorescently conjugated secondary antibody (goat anti-rabbit IRDye® 800CW [926–32211] 1:10,000 dilution) in Membrane Blocking Solution. The membranes were imaged with LI-COR® ODYSSEY CLx® imager. The bands were quantified relative to housekeeping protein levels using LI-COR® ODYSSEY CLx® Image Studio Ver 5.2 software. p-Akt/Akt ratio was assessed as previously described.
Hao X., Zhu B., Yang P., Dong D., Sahbaie P., Oliver P.L., Shen W.J., Azhar S, & Kraemer F.B. (2021). SNAP25 mutation disrupts metabolic homeostasis, steroid hormone production and central neurobehavior. Biochimica et biophysica acta. Molecular basis of disease, 1868(2), 166304.
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4

Investigating PI3K/AKT Signaling Pathway

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HP was purchased from Selleck (S5453, Houston, TX, USA) and dimethyl sulfoxide (DMSO, D4540) and thiazolyl blue tetrazolium bromide (MTT, M5655) were purchased from Sigma (St. Louis, MO, USA). Mouse monoclonal antibodies β-actin (BS6007M) was purchased from Bioworld Technology (Bloomington, MN, USA), and rabbit anti-AKT (9272), rabbit anti-PI3K (4249), and rabbit anti-phosphorylated AKT (P-AKT, 4060) polyclonal antibody from Cell Signaling Technology (Danvers, MA, USA). Mouse anti-BMP-7 (sc-53917) was purchased from Santa Cruz (Dallas, TX, USA) and rabbit anti-Cyclin D1 (ab226977) polyclonal antibody and rabbit anti-c-Myc (ab9106) polyclonal antibody from Abcam (Cambridge, UK).
Wei S., Sun Y., Wang L., Zhang T., Hu W., Bao W., Mao L., Chen J., Li H., Wen Y, & Chen Z. (2021). Hyperoside suppresses BMP-7-dependent PI3K/AKT pathway in human hepatocellular carcinoma cells. Annals of Translational Medicine, 9(15), 1233.
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5

Western Blot Antibody Dilution Protocol

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The following primary antibodies were diluted in TBST plus 5% nonfat dry milk for western blot: rabbit anti-mGBP-2 (1851 [21 (link),22 (link)]; 1:20,000), rabbit anti-actin (A2066; 1:3000) (Sigma), rabbit anti-α-tubulin (GTX102078; 1:10,000) (GeneTex, Irvine, CA, USA), mouse anti-GAPDH (60004; 1:20,000) (Proteintech, Rosemont, IL, USA). The above antibodies were incubated with membrane for 1 h at RT. The rabbit anti-RhoA (2117T; 1:4000) (Cell Signaling, Danvers, MA, USA), mouse anti-Rac1 (610650; 1:4000) (BD Biosciences, San Jose, CA, USA), mouse anti-CDC42 (610928; 1:250) (BD Biosciences), rabbit anti-Akt (9272; 1:3000) (Cell Signaling), and rabbit monoclonal anti-phosphoAkt (4060 (ser 473); 1:4000) (Cell Signaling) were incubated with membrane overnight at 4 °C. Hrp-conjugated goat anti-mouse immunoglobulin (1:8000) or goat anti-rabbit immunoglobulin (1:6000) (Jackson ImmunoResearch, West Grove, PA, USA) were diluted in TBST containing 5% w/v nonfat dry milk and incubated with the membranes for 1 h at RT.
Nyabuto G.O., Wilson J.P., Heilman S.A., Kalb R.C., Kopacz J.P., Abnave A.V, & Vestal D.J. (2021). The Large GTPase, GBP-2, Regulates Rho Family GTPases to Inhibit Migration and Invadosome Formation in Breast Cancer Cells. Cancers, 13(22), 5632.
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6

Antibody Sources for Neurobiological Analyses

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A rabbit polyclonal antiserum was raised against a cytosolic C-terminal PRG2 peptide (CAESYYRRMQARRYQD; Eurogentec). Antibodies were affinity-purified by Sulfolink column chromatography (Thermo Scientific). A rabbit antibody against PRG2 (AP5732c) from Abgent was also used in some experiments. The rabbit anti-PRG1 antibody was from R&D Systems (#MAB2874). Rabbit anti-PTEN (#9559), rabbit anti-AKT (#9272) and rabbit anti-pSer473AKT (#4060) were from Cell Signaling. Goat anti-PTEN (N19) was from Santa Cruz Biotechnology. Mouse anti-Flag antibody (F1804), mouse anti-MAP2 (M9942), mouse anti-α-tubulin (T6199) and anti-Flag Affinity Gel (A2220) was from Sigma. Chicken anti-GFP antibody (ab13970) was from Abcam, the mouse anti-Tau1 (MAB3420) and mouse anti-GAPDH (CB1001) from Millipore. Rabbit anti-Tubulin β-3 (Tuj1) (PRB-435P) was from Covance. HRP-conjugated anti-rabbit and anti-mouse secondary antibodies (#PI1000, #PI2000) were from Vector Labs and HRP-conjugated anti-chicken antibodies (G135A) from Promega. All fluorophore conjugated secondary antibodies were purchased from Jackson lab.
Brosig A., Fuchs J., Ipek F., Kroon C., Schrötter S., Vadhvani M., Polyzou A., Ledderose J., van Diepen M., Holzhütter H.G., Trimbuch T., Gimber N., Schmoranzer J., Lieberam I., Rosenmund C., Spahn C., Scheerer P., Szczepek M., Leondaritis G, & Eickholt B.J. (2019). The Axonal Membrane Protein PRG2 Inhibits PTEN and Directs Growth to Branches. Cell Reports, 29(7), 2028-2040.e8.
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7

Antibody Panel for Metabolic Signaling

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Rabbit anti-LDLR (ab30532), mouse anti-ABCA1 (ab18180), rabbit anti-AMPKα1 (ab32047), rabbit anti-AMPKα2 (ab3760), rabbit anti-INSIG1 (ab-70784), rabbit anti-CPT1α (ab83862), and mouse anti-Sik1 (ab64428) were purchased from Abcam Research Products, Cambridge, UK. Mouse anti-β tubulin (T7816) was purchased from Sigma Aldrich. Rabbit anti-SIRT1 (CY-P1016) was purchased from Cyclex. Rabbit anti-IRS1 (2382) was purchased from Cell Signaling Technology. Rabbit anti-IR (sc-711) was purchased from Santa Cruz Biotechnology. Rabbit anti-AKT (9272) and rabbit anti-phospho-AKT (9271) were purchased from Cell Signaling, mouse anti-PGC1α (ST1202-1SET) from Millipore, and mouse anti-HSP90 (no. 610418) from BD Bioscience.
Wagschal A., Najafi-Shoushtari S.H., Wang L., Goedeke L., Sinha S., deLemos A.S., Black J.C., Ramírez C.M., Li Y., Tewhey R., Hatoum I., Shah N., Lu Y., Kristo F., Psychogios N., Vrbanac V., Lu Y.C., Hla T., de Cabo R., Tsang J.S., Schadt E., Sabeti P.C., Kathiresan S., Cohen D.E., Whetstine J., Chung R.T., Fernández-Hernando C., Kaplan L.M., Bernards A., Gerszten R.E, & Näär A.M. (2015). Genome-wide identification of microRNAs regulating cholesterol and triglyceride homeostasis. Nature medicine, 21(11), 1290-1297.
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