Paint a gate pro software facscalibur

P3 ASCs were resuspended following digestion with 0.25% trypsin and washed three times with phosphate-buffered saline (PBS). A minimum of 1 × 10⁵ cells were collected from each sample. Samples were incubated with Anti-CD34 antibody (NBP2-29455; Novus), Anti-CD45RA antibody (561886; BD Pharmingen), Anti-CD73 antibody (orb629433; BIORBYT), Anti-CD90 antibody (561973; BD Pharmingen), and Anti-CD105 antibody (NB500-452; Novus) in the dark at room temperature for 30 min. The samples were subjected to flow cytometry, and data were analyzed using Paint-A-Gate Pro™ software (FACSCalibur™; BD Biosciences).

ASCs were resuspended following digestion with 0.25% trypsin and washed three times with phosphate-buffered saline (PBS). A minimum of 1 × 10⁵ cells were collected from each sample. Samples were incubated with phycoerythrin (PE)-coupled antibodies against rat CD29 (562,154; BD Biosciences), CD34 (sc-7324; Santa Cruz), CD45 (554,878; BD Biosciences), CD86 (551,396; BD Biosciences), and CD90 (551,401; BD Biosciences), and fluorescein isothiocyanate (FITC)-coupled antibody against rat CD44 (550,974; BD Biosciences) in the dark at room temperature for 30 min. Nonspecific isotype-matched antibodies were used as controls. The samples were subjected to flow cytometry, and data were analyzed using Paint-A-Gate Pro™ software (FACSCalibur™; BD Biosciences).