Beyozol rna isolation kit

As described in our pervious study [34 (link)], Total RNA was extracted and reverse transcripted into cDNA according to Beyozol RNA Isolation Kit and the All-in-one™ first-strand cDNA synthesis kit (Genecopoeia™, FulenGen), respectively. Quantitative PCR (qPCR) were carried out using the All-in-one™ qPCR mix (Genecopoeia™, FulenGen) according to the manufacturer’s instructions. Primer sequences used in this study were as followed: CDX2: forward:5′-CTCGGCAGCCAAGTGAAAACCA-3′, and reverse: 5′-GCTTTCCTCCGGATGGTGATGTA-3′ [35 (link)]; Villin: forward:5′-TCGGCCTCCAGTATGTAG-3′, and reverse: 5′- CGTCTTCGGGGTAGAACT-3′ [36 (link)]; MUC2: forward:5’-CAGGATGGCGCCTTCTGCTA-3’, and reverse:5’-ATGCTGCTCCAAGCTGAGGT-3’ [37 (link)]; TDO2: forward:5’-TCCTCAGGCTATCACTACCTGC-3’ and reverse: 5’-ATCTTCGGTATCCAGTGTCGG-3’; KMO: forward:5’-TGCCATCCCTCTAATTGGAGA-3’ and reverse: 5’-GCCCGCATTCATTCCTTGC-3’; KAT2: forward:5’- CACTTCAGTATTCTCCGAGTGC-3’ and reverse: 5’- AGCAGGTTCATCTAGGAGGAC-3’; UBC (internal control): forward:5’-ATTTGGGTCGCGGTTCTTG-3’ and reverse: 5’-TGCCTTGACATTCTCGATGGT-3’.

As described in our previous work [28 (link), 29 (link)], briefly, after treatment for 24 hr, Total RNA was extracted and reverse transcripted into cDNA according to Beyozol RNA Isolation Kit and the All-in-one™ first-strand cDNA synthesis kit (GenecopoeiaTM, FulenGen), respectively. Quantitative PCR (qPCR) was carried out using the All-in-one™ qPCR mix (GenecopoeiaTM, FulenGen) according to the manufacturer's instructions. Primer used in this study as followed: GPX4 forward, 5′-GAGGCAAGACCGAAGTAAACTAC-3′; reverse, 5′-CCGAACTGGTTACACGGGAA-3′; SLC7A11 forward, 5′-ACGGTGGTGTGTTTGCTGTCTC-3′; reverse, 5′-GCTGGTAGAGGAGTGTGCTTGC-3′; CDX2 forward, 5′-CTCGGCAGCCAAGTGAAAACCA-3′; reverse, 5′-GCTTTCCTCCGGATGGTGATGTA-3′; MUC2 forward, 5′-GAGGGCAGAACCCGAAACC-3′; reverse, 5′-GGCGAAGTTGTAGTCGCAGAG-3; GAPDH, forward, 5′-AACGGATTTGGTCGTATTGGG-3′, reverse, 5′-CCTGGAAGATGGTGATGGGAT-3′.

As described in our previous study, total RNA from the indicated treatment was extracted and converted into cDNA according to the Beyozol RNA Isolation Kit and the All-in-One™ first-strand cDNA synthesis kit (Genecopoeia™, FulenGen), respectively. Quantitative PCR (qPCR) was carried out to detect gene expression using the All-in-One™ qPCR mix (Genecopoeia™, FulenGen) according to the manufacturer's instructions. Primer sequences used in this study were listed as followed: GP2: forward: 5′-AATGTGCGGGAGAATGGTGT-3′ and reverse: 5′-TCTGAGCACTGGTTGACACT-3′; spib: forward: 5′-ATCACAGCTGCCACCATCTC-3′ and reverse: 5′-ACAGCTTAAGTGTGGGCCAT-3′; Marcksl1: forward:5′-GGAGAATGGCCACGTGAGAA-3′ and reverse: 5′-TCGATGGCATCACCAGTAGC-3′; SOX8: forward: 5′-ATCATTGGGCCAGGCATTGA-3′ and reverse: 5′-GTTGGGGAGGCTCTCCTTTC-3′; ANXA5: forward: 5′-GACCGACAGCATCATGGCTA-3′ and reverse: 5′-AGCATTGCTTCGGGATGTCA-3′; GAPDH: forward: 5′-TGTGTCCGTCGTGGATCTG-3′ and reverse: 5′-CCTGCTTCACCACCTTCTTGA-3′.