Rat tail collagen type I (#354236) and human fibronectin (#354008) were obtained from Corning. Mouse laminin I (#3400-010-01) was purchased from Trevigen, Inc. Recombinant human IgG1 Fc (#110-HG) and recombinant human semaphorin 3A Fc chimera (#1250-S3) were purchased from R&D Systems. A ROCK inhibitor (Y-27632; #688000) was purchased from EMD Millipore Corporation. Mouse anti-human FAK (pY397; #611806) was purchased from BD Transduction Laboratories. Phalloidin–Tetramethylrhodamine B isothiocyanate (#P1951) was obtained from Sigma-Aldrich. Alexa488 goat anti-mouse IgG (#115-545-003) was from Jackson ImmunoResearch Laboratories, Inc. CellTiter 96® AQueus One Solution (#G3580) was purchased from Promega Corporation. Characterized fetal bovine serum (#SH30071.03) and penicillin-streptomycin (#SV30010) were obtained from HyClone. All culture media was from Corning.
Celltiter 96 aqueus one solution
Manufactured by Promega
Sourced in United States
The CellTiter 96® AQueus One Solution is a colorimetric assay used for determining the number of viable cells in proliferation or cytotoxicity experiments. The assay contains a novel tetrazolium compound and an electron coupling reagent that produce a soluble, colored formazan product upon bioreduction by metabolically active cells.
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Lab products found in correlation
Rock inhibitor y 27632, by Merck Group (1 mentions)
Human fibronectin, by Corning (1 mentions)
Mouse laminin 1, by Bio-Techne (1 mentions)
Phalloidin tetramethylrhodamine b isothiocyanate, by Merck Group (1 mentions)
Rat tail collagen type 1, by Corning (1 mentions)
Alexa488 goat anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Macsquant vyb flow cytometer, by Miltenyi Biotec (1 mentions)
Propidium iodide, by Thermo Fisher Scientific (1 mentions)
2 protocols using celltiter 96 aqueus one solution
1
Characterization of Cell Adhesion Factors
2
Cell Proliferation and Cell Cycle Analysis
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Cell proliferation was evaluated using CellTiter 96AQueus One Solution (Promega, Madison, USA) and cell counting. For cell cycle analysis, NPM-ALK+ ALCL cells were washed with PBS and fixed overnight at 4°C in 70% ethanol. Cells were then washed with PBS supplemented with 0.1% BSA and incubated for 30 min with 10 μg/mL propidium iodide (Invitrogen, Waltham, USA) and 500 μg/mL of RNAse (Sigma-Aldrich, Saint Quentin Fallavier, France). Thereafter, cells were analyzed with a flow cytometer MACSQUANT VYB (Miltenyi, Paris, France).
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