Rabbit anti sqstm1

Manufactured by Merck Group

Rabbit anti-SQSTM1 is a laboratory reagent used in research applications. It is an antibody that specifically binds to the SQSTM1 protein, which is involved in cellular processes such as autophagy and protein trafficking. This product can be used in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to detect and study the SQSTM1 protein.

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Lab products found in correlation

Swine anti rabbit hrp, by Agilent Technologies (1 mentions) Bafilomycin a1 tlrl baf1, by InvivoGen (1 mentions) Rabbit anti atg7, by Cell Signaling Technology (1 mentions) Torin1, by Bio-Techne (1 mentions) Lps e coli o111 b4, by Merck Group (1 mentions) Rabbit anti akt, by Cell Signaling Technology (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Atp tlrl atpl, by InvivoGen (1 mentions) Rabbit anti p akt, by Cell Signaling Technology (1 mentions) Normal rabbit igg sc 2027, by Santa Cruz Biotechnology (1 mentions) Anti mouse 115 035 146, by Jackson ImmunoResearch (1 mentions) Protein a sepharose beads, by GE Healthcare (1 mentions) Lps tlrl eklps, by InvivoGen (1 mentions)

Spelling variants of this product

Rabbit anti-p62/SQSTM1 (P0067) (4 citations) Anti-SQSTM1 (2 citations) Rabbit anti- (2 citations) Rabbit anti-SQSTM1/p62 (2 citations)

2 protocols using rabbit anti sqstm1

Immunoblotting Techniques for Cellular Signaling

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All media (RPMI, DMEM) and PBS buffers were purchased from Gibco (61870–010; 41966–029; 14190–094). NaCl was purchased from Merck. LPS (E. coli O111:B4) and DPI were purchased from Sigma Aldrich (D2926). Torin1 was purchased from Tocris (4247); E-64d was obtained from Hycultec (HY-15282). Ionomycin (407952) was purchased from CalbioChem. ATP (tlrl-atpl) and bafilomycin A₁ (tlrl-baf1) were obtained from Invivogen. The prolyl-hydroxylase inhibitor DMOG was purchased from Cayman Chemical (71210). For immunoblotting, the following antibodies were used: rabbit anti-ACT (A2066; Sigma-Aldrich); rabbit-anti-HSP90A/B (Santa Cruz Biotechnology, sc-7947), rabbit anti-NFAT5 (Thermo Scientific, PA1-023), rabbit anti-MAP1LC3B (Novus Biologicals, NB100-2220), rabbit anti-ATG7 (Cell Signaling Technology, D12B11) rabbit anti-p-AKT (Cell Signaling Technology, 4060S), rabbit anti-AKT (Cell Signaling Technology, 9272S), rabbit anti-SQSTM1 (Sigma Aldrich, P0067), rabbit anti-HIF1A (Cayman Chemical, 10006421). As secondary antibody, we used swine anti-rabbit HRP (P0399, Dako).

Neubert P., Weichselbaum A., Reitinger C., Schatz V., Schröder A., Ferdinand J.R., Simon M., Bär A.L., Brochhausen C., Gerlach R.G., Tomiuk S., Hammer K., Wagner S., van Zandbergen G., Binger K.J., Müller D.N., Kitada K., Clatworthy M.R., Kurts C., Titze J., Abdullah Z, & Jantsch J. (2019). HIF1A and NFAT5 coordinate Na+-boosted antibacterial defense via enhanced autophagy and autolysosomal targeting. Autophagy, 15(11), 1899-1916.

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Immunoblot Analysis of Signaling Proteins

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Poly-L-lysine (P4832), dexamethasone/DEX (D4902-25MG), rabbit anti-ACTA1 (A2066) and rabbit anti-SQSTM1 (P0067) were purchased from Sigma. CpdA was synthesized according to the protocol described previously [49]. Chloroquine (sc-205,629), rabbit anti-NR3C1 (sc-8992x), mouse anti-LC3A/B (sc-398,822), rabbit anti-NFE2L2 (sc-13,032), rabbit anti-NFKBIA (C-21) and normal rabbit IgG (sc-2027) were from Santa Cruz Biotechnology. Horseradish peroxidase (HRP)-coupled secondary anti-rabbit (111–035-144) and anti-mouse (115–035-146) antibodies were purchased from Jackson ImmunoResearch. Rabbit anti-JUNB (C37F9), rabbit anti-ATG5 (2630), rabbit anti-p-MAPK/ERK (9101), mouse anti-MAPK/ERK (L34F12), mouse anti-p-MAPK/p38 (L28B10) and rabbit anti-MAPK/p38 (9212) were purchased from Cell Signalling Technology. Rabbit anti-HMOX1 (SPA-896) antibodies were from StressGen. Mouse anti-ubiquitin (VU-0101) antibodies were purchased from LifeSensors. Protein A Sepharose beads (17–5138-01) were from GE Healthcare Life Sciences. RNAse A (740,505) was from Macherey-Nagel. Proteinase K (19,131) was from Qiagen. LPS (tlrl-eklps) was purchased from Invivogen.

Mylka V., Deckers J., Ratman D., De Cauwer L., Thommis J., De Rycke R., Impens F., Libert C., Tavernier J., Vanden Berghe W., Gevaert K, & De Bosscher K. (2018). The autophagy receptor SQSTM1/p62 mediates anti-inflammatory actions of the selective NR3C1/glucocorticoid receptor modulator compound A (CpdA) in macrophages. Autophagy, 14(12), 2049-2064.

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