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Cd4 okt4 efluor450

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The CD4 (OKT4) eFluor450 is a fluorescently labeled monoclonal antibody that binds to the CD4 antigen expressed on the surface of T helper cells. It is designed for use in flow cytometry applications to identify and enumerate CD4+ T cells within a sample.

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Lab products found in correlation

Cd3 clone sp34 2 al700, by BD (2 mentions) Hladr g46 6 apc h7, by BD (2 mentions) Il 2 mq1 17h12 fitc, by BD (2 mentions) Ki67 b56 fitc, by BD (2 mentions) Tnfa mab11 pe cy7, by Thermo Fisher Scientific (2 mentions) Cd28 cd28.2 ecd, by Beckman Coulter (2 mentions) Cd95 dx2 pe cy5, by BioLegend (2 mentions) Live dead fixable aqua dead cell stain, by Thermo Fisher Scientific (2 mentions) Lsrfortessa, by BD (2 mentions) Cytofix cytoperm, by BD (2 mentions) Facsaria, by BD (2 mentions)

2 protocols using cd4 okt4 efluor450

1

Multiparametric Cytometry of Immune Cells

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Polychromatic flow cytometry and cell sorting were performed on stained mononuclear cells utilizing the BD LSRFortessa and FACS Aria, respectively, equipped with FACS DiVA software. The following Abs were used for staining at predetermined concentrations: CD3 (clone SP34-2) AL700, HLADR (G46-6) APC-H7, IL-2 (MQ1-17H12) FITC, and Ki67 (B56) FITC from BD; CD4 (OKT4) eFluor450, CD8 (SK1) PerCP-e710, IFNg (4S.B3) eFluor450, IL17A (eBio64DEC17) PE, IL22 (IL22JOP) APC, and TNFa (MAb11) PE-Cy7 from eBioscience; CD28 (CD28.2) ECD from Beckman Coulter; and CD95 (DX2) PE-Cy5 from Biolegend. Cell viability was assessed using the Live/Dead Aqua Fixable Dead Cell Stain (Invitrogen). Cells were permeabilized with Cytofix/Cytoperm (BD) prior to intracellular staining. Acquired data were analyzed using FlowJo software. To quantify cell subset frequencies, we used a threshold cutoff of 100 cells of the parent population. For TH17 functional analyses, cytokine gates were established on a threshold cutoff of 100 CD4+ or CD8+ TM and Boolean gates utilized to ascertain frequencies – populations not meeting this threshold were not considered for analysis.
Ortiz A.M., Klase Z.A., DiNapoli S.R., Vujkovic-Cvijin I., Carmack K., Perkins M.R., Calantone N., Vinton C.L., Riddick N.E., Gallagher J., Klatt N.R., McCune J.M., Estes J.D., Paiardini M, & Brenchley J.M. (2015). IL-21 and Probiotic Therapy Improve TH17 Frequencies, Microbial Translocation, and Microbiome in ARV-Treated, SIV-Infected Macaques. Mucosal immunology, 9(2), 458-467.
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2

Multiparametric Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polychromatic flow cytometry and cell sorting were performed on stained mononuclear cells utilizing the BD LSRFortessa and FACS Aria, respectively, equipped with FACS DiVA software. The following Abs were used for staining at predetermined concentrations: CD3 (clone SP34-2) AL700, HLADR (G46-6) APC-H7, IL-2 (MQ1-17H12) FITC, and Ki67 (B56) FITC from BD; CD4 (OKT4) eFluor450, CD8 (SK1) PerCP-e710, IFNg (4S.B3) eFluor450, IL17A (eBio64DEC17) PE, IL22 (IL22JOP) APC, and TNFa (MAb11) PE-Cy7 from eBioscience; CD28 (CD28.2) ECD from Beckman Coulter; and CD95 (DX2) PE-Cy5 from Biolegend. Cell viability was assessed using the Live/Dead Aqua Fixable Dead Cell Stain (Invitrogen). Cells were permeabilized with Cytofix/Cytoperm (BD) prior to intracellular staining. Acquired data were analyzed using FlowJo software. To quantify cell subset frequencies, we used a threshold cutoff of 100 cells of the parent population. For TH17 functional analyses, cytokine gates were established on a threshold cutoff of 100 CD4+ or CD8+ TM and Boolean gates utilized to ascertain frequencies – populations not meeting this threshold were not considered for analysis.
Ortiz A.M., Klase Z.A., DiNapoli S.R., Vujkovic-Cvijin I., Carmack K., Perkins M.R., Calantone N., Vinton C.L., Riddick N.E., Gallagher J., Klatt N.R., McCune J.M., Estes J.D., Paiardini M, & Brenchley J.M. (2015). IL-21 and Probiotic Therapy Improve TH17 Frequencies, Microbial Translocation, and Microbiome in ARV-Treated, SIV-Infected Macaques. Mucosal immunology, 9(2), 458-467.
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