Percp cy5.5 pd 1

Manufactured by BioLegend

Sourced in United States

PerCP/Cy5.5-PD-1 is a fluorescent-conjugated antibody used for the detection and analysis of the programmed cell death protein 1 (PD-1) marker. It is designed for flow cytometry applications.

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Close spelling variants of this product

PD-1-PerCP/Cy5.5 (EH12.2H7, Anti-PD-1 PerCP-Cy5.5 (EH12.2H7, PerCP/Cy5.5 anti-human CD279/PD-1 PD-1-PerCP-Cy5.5 PerCP-Cy5.5

2 protocols using percp cy5.5 pd 1

Phenotypic Characterization of PBMCs

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Human PBMCs were resuspended with 200 μL PBS buffer and incubated with fluorochrome-conjugated antibodies, anti-human Krome Orange-CD4, Alexa Fluor 750-CD45RA, ECD-CD25 (Beckman Coulter Life Science, United States), Pacific blue™-CXCR5, PerCP/Cy5.5-PD-1, APC-TIM-3, Brilliant violet 650™-LAG-3, PE/Cy7-CTLA-4, FITC-CCR6, and PE-CXCR3 (BioLegend, United States), for 20 min at room temperature, and matched isotype controls were performed in this study according to the manufacturer’s instructions. The samples were detected by the CytoFLEX Flow Cytometer, and the data were analyzed by CyExpert software (Beckman Coulter, United States).

Cui D., Jiang D., Yan C., Liu X., Lv Y., Xie J, & Chen Y. (2022). Immune Checkpoint Molecules Expressed on CD4+ T Cell Subsets in Chronic Asymptomatic Hepatitis B Virus Carriers With Hepatitis B e Antigen-Negative. Frontiers in Microbiology, 13, 887408.

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Peptide Library Synthesis and Liposome Formulation

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Trp2, AH1 and WT1 peptide libraries and peptides were synthesized by GenScript. CoPoP was synthesized as previously reported (32 (link)). The following lipids were used to produce liposomes: dioleoylphosphatidylcholine (DOPC; Corden #LP-R4–070), cholesterol (PhytoChol; Wilshire), and synthetic PHAD-3D6A (Avanti; #699855). QS-21 was purchased from Desert King (part number NC0949192). The APC-CD8a (Clone: CT-CD8a) antibody was obtained from Accurate Chemical and Scientific Corporation (#ACL168APC). The following antibodies were obtained from BioLegend: APC-CD8a (Clone: CT-CD8a; #100712), FITC-CD4 (Clone: GK1.5; #100405), AF700-CD45 (Clone: 30-F11; #103127), APC-Cy7-CD44 (Clone: IM7; #103027), PE/Cy7-CD62L (Clone: MEL-14; #104417), PerCP-Cy5.5-PD-1 (Clone: RMP1–30; #109119), pacific blue-IFN-γ (Clone: XMG1.2; #505818), BV605-TNF-α (Clone: MP6-XT22; #506329), PE/Cy7-Granzyme B (Clone: QA16A02; #372213). Anti-mouse PD-1 (#BP0146) and anti-mouse CTLA-4 (#BP0131) were acquired from Bio X Cell. Other reagents used were Golgiplug/Brefeldin A (BD; #555029), Live/Dead dye (Invitrogen; #L34857), Fc-block (Clone: 2.4G2; BD #553142), fixation/permeabilization kit (BD #554714), red blood cell (RBC) lysis buffer (BioVision #5830), Collagenase Type I (Gibco #17018–029), and DNase I (Roche #04536282001).

He X., Zhou S., Quinn B., Jahagirdar D., Ortega J., Long M.D., Abrams S.I, & Lovell J.F. (2022). An In Vivo Screen to Identify Short Peptide Mimotopes with Enhanced Antitumor Immunogenicity. Cancer immunology research, 10(3), 314-326.

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