On the 42nd day of the experiment, the feeders of the pigs were emptied four hours before blood sampling. Samples (4 mL) of blood were collected from the jugular veins of six animals per group and deposited into vacutainer tubes containing ethylenediaminetetraacetic acid as an anticoagulant agent. The hematological parameters (white blood cells, lymphocytes, monocytes, granulocytes, red blood cells, hematocrit, hemoglobin, and thrombomodulin), were analyzed with an “MS4 automated analyzer” (Melet Schloesing Lab, Osny, France) [38 (link)]. Additionally, some serum biochemical parameters (albumin, alanine aminotransferase, aspartate aminotransferase, cholesterol, creatine kinase, glucose, total bilirubin, and triglycerides) were determined with an “IDEXX VETTEST 8008 analyzer” (IDEXX LAB, Westbrook, ME, USA) [39 (link)].
Vettest 8008 analyzer
Manufactured by IDEXX
Sourced in United States
The VETTEST 8008 analyzer is a biochemical analyzer designed for veterinary use. It provides quantitative analysis of various analytes in animal blood samples. The instrument is capable of performing a range of clinical chemistry tests to support veterinary diagnostics and patient care.
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Lab products found in correlation
4 protocols using vettest 8008 analyzer
1
Hematological and Biochemical Analysis of Pigs
2
Blood Plasma Biochemical Analysis
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Blood was drawn from the vena caudalis and samples stabilized with 40 IU sodium heparin per 1 mL. Blood was separated by centrifugation at 12 000 ×g for 10 min at 4°C, and plasma samples were held at −80°C until analysis. The following biochemical indices were evaluated: aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and creatine kinase (CK). For the biochemical analysis of plasma, the VETTEST 8008 analyzer (IDEXX Laboratories Inc., USA) was used.
3
Biochemical Profile Analysis with VETTEST 8008
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A VETTEST 8008 analyzer (IDEXX Laboratories Inc., USA) was used according to the manufacturer's instructions to determine biochemical indices, including glucose (GLU), total proteins (TP), ammonia (NH 3 ), aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin (ALB), creatine (CREA), lactate dehydrogenase (LDH), creatine kinase (CK), lactate (LACT), phosphorous (PHOS), magnesium (Mg), triglyceride (Trig), alkaline phosphatase (ALP), and calcium (Ca 2+ ).
4
Serum Biochemical Profiling and Oxidative Stress Assessment
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Serum biochemical indices including total protein (TP), triglyceride (TG), total cholesterol (CHO), glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were measured using kits with a VET-TEST 8008 analyzer (IDEXX Laboratories Inc., Maine, USA). Superoxide dismutase (SOD) activity was determined using a SOD Assay Kit (Sigma, 19160) . Commercial assay kits were used for measurement of catalase (CAT) activity (ThermoFisher Scienti c, CA, USA) and malondialdehyde (MDA) concentration (Sigma-Aldrich, Missouri, USA). A turbidimetric method was used for determination of lysozyme activity according to Swain et al. [21] . MPO activity was determined as described by Quade and Roth [22] . Serum antiprotease activity was quanti ed based on Ellis [23] with some modi cations [24] .
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