Anti dykddddk tag antibody

Paraffin section slides were stained with anti-Her2/ERBB2 antibody (Sino Biological #10004-T56) to detect Her2 expression on liver cell surface or stained with H&E for the verification of inflammatory infiltration. T cells and CAR +T cells were stained with anti-CD3 antibody (Abcam #11089) and purified anti-DYKDDDDK tag antibody (Biolegend #637301) to perform immunofluorescence. Paraffin section slides of tumor tissues were stained with antipimonidazole monoclonal antibody (Hypoxyprobe, #HP1) and goat antimouse secondary antibody (Maokang Biotechnology, Shanghai), #MM51006) to detect hypoxic region in tumors by immunofluorescence. For staining quantification, slides were scanned digitally using TissueFAXS 200 (TissueGnostics).

Staining of the different cell types with either anti-hACE2.01-16 (generated by us)/Purified anti-DYKDDDDK Tag Antibody (Cat#637302, BioLegend), /commercial anti-hACE2 (#ABIN1169449, antibodies-online) were performed at 4 °C for 1 h, cells were then washed in FACS buffer (1% BSA and 0.05% Sodium Azide in PBS) and secondary antibody (Alexa Fluor 647-conjugated Goat Anti-Mouse IgG, Cat#115-606-062, Jackson ImmunoResearch Laboratories) was added for 30 min at 4 °C. Then, cells were washed in FACS buffer followed by CytoFlex analysis. Data were analyzed using FCS Express 6/7.