The acetonitrile, acetone, and sodium chloride were provided by Honeywell (Charlotte, NC, USA). Petroleum ether, magnesium sulfate anhydrous, sodium sulfate anhydrous, trisodium citrate dihydrate (Na3C6H5O7·2H2O), were from Chempur (Piekary Śląskie, Poland), and disodium hydrogen citrate sesquihydrate (Na2HC6H5O7·1.5H2O) was purchased from Sigma-Aldrich (Burlington, MA, USA). A primary secondary amine (PSA, a polymerically bonded ethylenediamine-N-propyl phase that contains both primary and secondary amines, 50 µm particle size, 70 Å pore size), internal standard triphenyl phosphate (TPP, molecular formula: C18H15O4P, molecular weight: 326.3 g/mol, CAS No: 115–86-6), and fluopyram were from Supelco (Bellefonte, PE, USA), and tebuconazole was supplied by Instytut Przemysłu Organicznego (Warsaw, Poland). The purity of all standards was above 98%. Fungicide standards at the concentration of 1000 µg/mL were prepared in acetone and stored at − 17 °C. Working solutions were prepared by dissolving appropriate amounts of the standard solution in Petroleum ether and stored at 4 °C.
Sodium sulfate anhydrous
Manufactured by Chempur
Sourced in Poland
Sodium sulfate anhydrous is a chemical compound with the formula Na2SO4. It is a white, crystalline solid that is soluble in water. Sodium sulfate anhydrous is commonly used as a drying agent and in various industrial and laboratory applications.
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Lab products found in correlation
Trisodium citrate dihydrate, by Chempur (1 mentions)
Petroleum ether, by Chempur (1 mentions)
Acetone, by Honeywell (1 mentions)
Acetonitrile, by Honeywell (1 mentions)
Hydrochloric acid (hcl), by Chempur (1 mentions)
Acetic acid, by Chempur (1 mentions)
Ethyl acetate, by Chempur (1 mentions)
Methanol, by Chempur (1 mentions)
Mueller hinton broth (mhb), by BD (1 mentions)
Difco nutrient broth, by BD (1 mentions)
Crystal violet, by Chempur (1 mentions)
Alamarblue cell viability reagent, by Thermo Fisher Scientific (1 mentions)
Sytox green nucleic acid stain, by Thermo Fisher Scientific (1 mentions)
Iron 3 chloride, by Merck Group (1 mentions)
Diethyl ether, by Chempur (1 mentions)
Isobornyl methacrylate, by Merck Group (1 mentions)
Thionyl chloride, by Thermo Fisher Scientific (1 mentions)
Methylene chloride, by Chempur (1 mentions)
Toluene, by EKF Diagnostics (1 mentions)
N octanol, by Merck Group (1 mentions)
4 protocols using sodium sulfate anhydrous
1
Multiresidue Fungicide Extraction Protocol
2
Antimicrobial Activity Evaluation
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Pseudomonas putida (DSM 291), Pseudomonas moorei (DSM 12647), Sphingomonas mali (DSM 10565) and Bacillus subtilis (DSM 3657) were obtained from the German Collection of Microorganisms and Cell Cultures GmbH (Germany). Methylisothiazolinone, chloroxylenol, 3-indoleacetic acid, iron(III) chloride, phospholipid standards and ammonium format were purchased from Merck (Poland). SYTOX™ Green Nucleic Acid Stain, General Oxidative Stress Indicator (CM-H2DCFDA, 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate, acetyl ester) and AlamarBlue Cell Viability Reagent were obtained from Thermo Fisher Scientific (Poland). Difco™ nutrient broth and Mueller Hinton broth were purchased from Becton Dickinson (Poland). DMSO and phosphate-buffered saline (PBS) were obtained from BioShop (Canada). Methanol, ethyl acetate, hydrochloric acid, acetic acid, crystal violet and sodium sulfate anhydrous were obtained from Chempur (Poland). All of the other reagents with a high analytical purity grade were purchased from Avantor (Poland).
3
Synthesis and Characterization of Ibuprofen Derivatives
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All used in this research reagents were commercially available materials and were used without further purification. (R,S)-Ibuprofen (>98%) was provided from Am-Beed (Arlington Heights, IL, USA). Thionyl chloride (99.5%) was obtained from Acros Organics (Geel, Belgium). l -Histidine (≥99%) and l -phenylalanine (>98.5%) were purchased from Carl Roth (Karlsruhe, Germany). l -Tyrosine, acetonitrile (≥99.9%) for HPLC gradient grade, deuterated DMSO (99.9%), 2-ethylhexyl acrylate (2-EHA) (98%), isobornyl methacrylate (IBOMA) (>99%), azobis(isobutyronitrile) (AIBN) (99%), and n-octanol (≥99%) were provided by Sigma-Aldrich (Steinheim am Albuch, Germany). l -Tryptophan was provided by Tokyo Chemical Industry Co., Ltd (TCI, Tokyo, Japan). Propanol (PrOH), ammonium hydroxide solution 25% (NH3·H2O), anhydrous sodium sulfate, n-hexane, methylene chloride, and diethyl ether were high-purity obtained from Chempur (Gliwice, Poland). 4-Acryloyloxy benzophenone (ABP) was obtained from POLY-CHEM BmbH (Bitterfeld-Wolfen, Germany). Acrylic acid (AA) (>98%), ethanol (EtOH), chloroform, toluene, and ethyl acetate were of analytical grade purchased from StanLab (Lublin, Poland). Deuterated chloroform (CDCl3) (99.8%) (+0.03% TMSCl) was purchased from Eurisotop (Cheshire, England).
4
Quantifying Organic Compounds: A Rigorous Approach
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Methanol and n-hexane of high purity were purchased from Honeywell Specialty Chemicals Seelze GmbH (Hannover, Germany). L-ascorbic acid and anhydrous sodium sulfate were obtained from Chempur (Piekary Śląskie, Poland), and potassium hydroxide was purchased from POCH (Zabrze, Poland). Water for sample preparations was purified through SolPure XIO P (ELKAR, Kęty, Poland).
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