Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody

Ivalin (>98%) was isolated from Carpesium divaricatum and identified by ESI-MS, ¹H, and ¹³C NMR data [16 (link)]. Compound were dissolved at 10 μM in dimethyl sulfoxide (DMSO) as a stock solution and diluted to desired concentrations according to the research requirement. 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) and 4,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Antibodies against phosphor-Cdc2, Cdc2, Cdc25A, Cyclin B1, Bcl-2, and Bax were purchased from Cell Signaling Technology (CST, Inc., Beverly, MA, USA). Tubulin antibody was purchased from Proteintech Group, Inc. (Chicago, IL, USA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was purchased from Abcam, Inc. (Cambridge, MA, USA). We bought the Annexin V–fluoresceine isothiocyanate (FITC) Apoptosis Detection Kit from BD Biosciences (San Jose, CA, USA). Propidium iodide (PI) and RIPA lysis buffer were obtained from the Beyotime Institute of Biotechnology (Shanghai, China). Vincristine (VCR) was purchased from Shenzhen Main Luck Pharmaceuticals Inc. (Shenzhen, China).

Sample proteins underwent separation via agarose- sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), followed by transfer onto polyvinylidene fluoride (PVDF) membranes. Next, the membranes were blocked using 3% bovine serum albumin (BSA) in phosphate buffered saline (PBS) and probed with CXCL13 antibody (Abcam, Cambridge, UK) or the Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Abcam, Cambridge, UK) as the primary antibody. Besides, anti-rabbit immunoglobulins (Abcam, Cambridge, UK) were utilized as horseradish peroxidase-conjugated secondary antibodies. In addition, semi-quantitative analysis of the enhanced chemiluminescence (ECL) western blot detection system was employed for the visualization of specific proteins.