Bronchial rings were connected to isometric force transducers Fort25 (WPI, UK). The signal was amplified by a Powerlab 8/36 and Octal Bridge Amp system (AD instruments, UK), recorded and analyzed via the LabChart 7 interface software (AD instruments, UK). Tissues were mounted on hooks and one was attached with thread to a stationary rod and the other hook tied with thread to an isometric force displacement transducer. Airways were allowed to equilibrate by flushing with fresh KH buffer solution. Passive tension was determined by gentle stretching of tissue (0.5–1.0 g) during equilibration. The isometric change in tension was measured by the transducer and the tissue vitality was assessed by EFS10Hz. These procedures allowed the bronchial rings to be correctly positioned between the hooks. When the passive contractile tone reached the plateau, rings were washed three times with fresh KH buffer solution and allowed to equilibrate for further 45 min.
Octal bridge amp system
Manufactured by ADInstruments
Sourced in United Kingdom
The Octal Bridge Amp system is a versatile lab equipment designed to amplify and condition various signal types. It features eight independent bridge amplifier channels that can be used for a range of applications requiring precise signal amplification and conditioning.
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Lab products found in correlation
3 protocols using octal bridge amp system
1
Bronchial Ring Isometric Tension Measurement
2
Bronchial Tissue Isometric Force Measurement
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Bronchial rings were connected to isometric force transducers Fort25 (WPI, UK). PowerLab 8/36 and Octal Bridge Amp system (ADInstruments, UK), recorded and analyzed with the LabChart 7 interface software (ADInstruments, UK). Tissues were mounted on hooks, and attached with thread to a stationary rod and the other tied with thread to an isometric force displacement transducer. Airways were allowed to equilibrate by flushing with fresh KH buffer solution. Passive tension was determined by gentle stretching of tissue (0.5–1.0 g) during equilibration. The isometric change in tension was measured by the transducer and the tissue vitality assessed by transmural stimulation (also called electrical field stimulation, EFS) at 25 Hz; when the passive contractile tone reached the plateau, rings were washed three times with KH buffer solution and allowed to equilibrate for 45 min [35 , 36 (link)].
3
Isometric Tension Measurement in Bronchial Rings
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Bronchial rings were connected to isometric force transducers Fort25 (WPI, UK). The signal was amplified by PowerLab 8/36 and Octal Bridge Amp system (ADInstruments, UK), recorded and analysed using the LabChart 7 interface software (ADInstruments, UK). Tissues were mounted on hooks and attached with a thread to a stationary rod and the other end was tied with a thread to an isometric force displacement transducer. Airways were allowed to equilibrate by flushing with fresh KH buffer solution. Passive tension was determined by gentle stretching of tissue (0.5-1.0 g) during equilibration. The isometric change in tension was measured by the transducer. The tissue vitality and maximal contractile responsiveness was assessed by acetylcholine at a 100 μM concentration and/or by transmural stimulation (also called electrical field stimulation [EFS]) at 25 Hz. These procedures allowed the bronchial rings to be correctly positioned between the hooks. When the response reached a plateau, the rings were washed thrice and allowed to further equilibrate [12 , 14 (link), 17 (link)].
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