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Fitc annexin 5 staining

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FITC-annexin V is a fluorescent reagent used to detect apoptosis (programmed cell death) in cells. It binds to the phospholipid phosphatidylserine, which is exposed on the surface of cells undergoing apoptosis. This allows the identification and quantification of apoptotic cells using flow cytometry or fluorescence microscopy.

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Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)

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Annexin V-FITC (1630 citations) Annexin V (870 citations) Annexin V staining (19 citations) Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining (7 citations) Annexin V-FITC/PI dual staining kit (6 citations) Annexin V (Annexin V-FITC) (5 citations) FITC-labeled annexin V and PI staining (4 citations) Annexin V-FITC/propidium iodide (PI) Double Staining Kit (3 citations) Annexin V-FITC staining solution (3 citations) Annexin V-Fluorescein Isothiocyanate (FITC)/Propidium Iodide (PI) Staining kit (3 citations)

3 protocols using fitc annexin 5 staining

1

Quantifying Apoptosis and Necrosis

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Apoptotic cells were measured based on nuclear staining with PI46 (link). Briefly, the cells were incubated for 0.5 h in a hypotonic PI-staining buffer (0.1% sodium citrate, 0.1% Triton X-100, and 50 μg/ml PI) and then subjected to flow cytometry to quantify the apoptotic/necrotic cell population (sub-G1/G0 cell fraction). Early-stage apoptosis was determined by FITC-annexin V staining (BD Biosciences), based on the translocation of phosphatidylserine to the extracellular membrane leaflet in apoptotic cells.
Zou Q., Jin J., Hu H., Li H.S., Romano S., Xiao Y., Nakaya M., Zhou X., Cheng X., Yang P., Lozano G., Zhu C., Watowich S.S., Ullrich S.E, & Sun S.C. (2014). USP15 stabilizes MDM2 to mediate cancer cell survival and inhibit antitumor T cell responses. Nature immunology, 15(6), 562-570.
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2

Quantifying Apoptosis and Necrosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Apoptotic cells were measured based on nuclear staining with PI46 (link). Briefly, the cells were incubated for 0.5 h in a hypotonic PI-staining buffer (0.1% sodium citrate, 0.1% Triton X-100, and 50 μg/ml PI) and then subjected to flow cytometry to quantify the apoptotic/necrotic cell population (sub-G1/G0 cell fraction). Early-stage apoptosis was determined by FITC-annexin V staining (BD Biosciences), based on the translocation of phosphatidylserine to the extracellular membrane leaflet in apoptotic cells.
Zou Q., Jin J., Hu H., Li H.S., Romano S., Xiao Y., Nakaya M., Zhou X., Cheng X., Yang P., Lozano G., Zhu C., Watowich S.S., Ullrich S.E, & Sun S.C. (2014). USP15 stabilizes MDM2 to mediate cancer cell survival and inhibit antitumor T cell responses. Nature immunology, 15(6), 562-570.
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3

Evaluating IL-25 Effects on HCC Cell Lines

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After treatment with IL-25 or co-culture with IL-25-stimulated macrophages, the cell growth, migration and invasion of HCC cell lines (MHCC97L and HepG2) were evaluated by Cell Counting Kit-8 (CCK-8) (Dojindo, Japan), Brdu staining (Sigma-Aldrich) and Transwell assays. The cell apoptosis of HCC cell lines were evaluated after treatment with IL-25 using FITC Annexin V staining (BD Biosciences). Please see the Additional file 1 for details.
Li Q., Ma L., Shen S., Guo Y., Cao Q., Cai X., Feng J., Yan Y., Hu T., Luo S., Zhou L., Peng B., Yang Z, & Hua Y. (2019). Intestinal dysbacteriosis-induced IL-25 promotes development of HCC via alternative activation of macrophages in tumor microenvironment. Journal of Experimental & Clinical Cancer Research : CR, 38, 303.
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