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Cd11b antibody ab133357

Manufactured by Abcam
Sourced in United States

The CD11b antibody [ab133357] is a mouse monoclonal antibody that recognizes the CD11b antigen. CD11b is a cell surface glycoprotein that is expressed on the surface of myeloid cells, including monocytes, macrophages, and granulocytes. The antibody can be used for the identification and characterization of CD11b-positive cells in various applications.

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2 protocols using cd11b antibody ab133357

1

Peptide-Mediated Fluorescent Assay

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PT-peptide (LDHVCNFRVMPRLRSWELYFRGDVWCPGWTVIKG) and FITC-labeled PT-peptide (labeled on N-terminal) were synthesized from LTK BioLaboratories Inc. (Tauyuan, Taiwan). Trypan blue, RPMI 1640 medium, L-glutamine, and sodium pyruvate were purchased from GIBCO (Grand Island, NY, USA). Dichloro-dihydro-fluorescein diacetate (DCFH-DA) and RIPA lysis buffer were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Potassium dihydrogen phosphate (KH2PO4) and dipotassium hydrogen phosphate (K2HPO4) were purchased from Merck Co. Ltd.(Darmstadt, Germany). Fetal bovine serum (FBS) was purchased from Hyclone (Logan, UT, USA). GPADH antibody [ab8245] and CD11b antibody [ab133357] were purchased from Abcam (Cambridge, MA, USA).
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2

LPS-Induced Inflammation: Lipid Mediators Analysis

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LPS (from E. coli O26 by phenol extraction) was purchased from FUJIFILM Wako (Tokyo, Japan) . LPC18:1 and LPE18:1 were purchased from Avanti Polar Lipids (Alabaster, AL, USA) . CD11b antibody (ab133357) and apocynin were purchased from Abcam (Cambridge, MA, USA) . Iba1 antibody (MABN92) was purchased from Sigma-Aldrich (St. Louis, MO, USA) . β-actin (sc-47778) antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) .
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