The 4 T1 cells were cultured cells in a 96-well plate and incubated in a CO2 incubator overnight at 37 °C for 24 h. The spent medium was aspirated, and cells were washed with phosphate buffer saline (PBS) (#TL1006, Himedia, India). After removing the PBS, Ephedra foeminea extract at its IC50 values (40.09 µg/ml) or standard drug-camptothecin (8.5 µM) was added and incubated for 24 h. The cells were then stained with acridine orange (A1301, Thermo Fischer, USA) and ethidium bromide (17989, Thermo Fischer, USA) and incubated for 15 min at room temperature. Dual fluorescent staining solution (10 μl) containing 5 μg/ml AO and 5 μg/ml EtBr was used to find out live and dead cells. Five hundred cells were counted within 20 min and the morphology of apoptotic cells were examined to detect the percentage of the apoptotic cells (Liu et al., 2015b (link)). The cells were imaged under confocal microscopy (Zeiss, Germany), and data analysis was done using ImageJ software.
Tl1006
Manufactured by HiMedia
Sourced in India
The TL1006 is a laboratory instrument designed for general-purpose applications. It features a compact and durable construction, making it suitable for use in various laboratory environments. The core function of the TL1006 is to perform accurate measurements and analysis tasks.
Automatically generated - may contain errors
4 protocols using tl1006
1
Apoptosis Assay of T1 Cells
2
HEK293T Cell Lysis and Protein Extraction
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HEK293T cells were grown in DMEM (AL007A, Himedia) supplemented with 10% FBS (RM1112, Himedia) and antibiotic-antimycotic agent (15,240,062, Gibco). Cells were collected and washed with PBS (TL1006, Himedia). Washed cell pellet was lysed in RIPA buffer (150 mM NaCl, 5 mM EDTA, 50 mM Tris, 1% NP40 (IGEPAL), 0.1% Na-deoxycholate and 0.1% SDS) containing Halt protease and phosphatase inhibitor cocktail (PI78441, Invitrogen).
3
HEK293T Cell Lysis Protocol
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Cell culture and cell lysate preparation HEK293T cells were grown in DMEM (AL007A, Himedia) supplemented with 10% FBS (RM1112, Himedia) and antibiotic-antimycotic agent (15240062, Gibco). Cells were collected and washed with PBS (TL1006, Himedia). Washed cell pellet was lysed in RIPA buffer (150 mM NaCl, 5 mM EDTA , 50 mM Tris, 1% NP40 (IGEPAL), 0.1% Na-deoxycholate and 0.1% SDS) containing Halt protease and phosphatase inhibitor cocktail (PI78441, Invitrogen).
4
HEK293T Cell Lysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell culture and cell lysate preparation HEK293T cells were grown in DMEM (AL007A, Himedia) supplemented with 10% FBS (RM1112, Himedia) and antibiotic-antimycotic agent (15240062, Gibco). Cells were collected and washed with PBS (TL1006, Himedia). Washed cell pellet was lysed in RIPA buffer (150 mM NaCl, 5 mM EDTA , 50 mM Tris, 1% NP40 (IGEPAL), 0.1% Na-deoxycholate and 0.1% SDS) containing Halt protease and phosphatase inhibitor cocktail (PI78441, Invitrogen).
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