The largest database of trusted experimental protocols

6 protocols using sodium dodecyl sulfate (sds)

1

Reagents for Oxidative Stress Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
MDZ was purchased from Yichang Renfu Pharmacy Company (Yichang, China). Diazepam was purchased from Shandong Xinyi Pharmaceutical Co., Ltd. (Dezhou, China). Poloxamer 407 (P407), poloxamer 188 (P188), Macrogol 4000 (PVP K30), and Sodium dodecyl sulfate (SDS) were purchased from BASF, Ludwigshafen, Germany. Polysorbate 80 (TW80), polysorbate 20 (TW20), and carboxymethylcellulose sodium (CMC-Na) were ordered from Sinopharm Chemical Reagent Co. (Beijing, China). Sodium dodecyl sulfate was procured from VWR International (Radnor, PA, USA). Hydroxypropyl methylcellulose (HPMC) was purchased from Dow Chemical Company (Michigan, MI, USA). Dioctyl sodium sulfosuccinate (DOSS) was purchased from Hunan HuaNa Pharmaceutical Factory Chiral Pharmaceutical Co., Ltd. (Hunan, People’s Republic of China). HPLC-grade acetonitrile and methanol were obtained from Thermo Fisher Scientific (Waltham, MA, USA). All other reagents were of analytical grade. The purified water used in this study was prepared using a Mille-Q system (EMD Millipore, Billerica, MA, USA). The Cu/Zn-SOD assay kit with WST-8, the GSH assay kit, the lipid peroxidation MDA assay kit, and the BCA protein assay kit were purchased from Shanghai Biyuntian Biotechnology Co., Ltd. (Shanghai, China).
+ Open protocol
+ Expand
2

Evaluation of Cyclosporin Formulations

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cyclosporin A (CsA) was purchased from Taishang Chemical Pharmacy Company (Taishang, China). Cyclosporin D (CsD) was purchased from Shanghai Tongtian Biotechnology Company (Shanghai, China). Neoral® (Ciclosporin soft gelatin capsules) was purchased from local drugstore. Vitamin E polyethylene glycol succinate (TPGS) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Sodium dodecyl sulfate (SDS) were purchased from BASF, Ludwigshafen, Germany. Hydroxypropyl cellulose (HPC) was purchased from Nippon Soda Co., Ltd. (Tokyo, Japan). Simulated intestinal uid (SIF) and simulated gastric uid (SGF) were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). Roswell Park Memorial Institute (RPMI) 1640 medium, phosphate buffered saline (PBS) and Hank's Balanced Salt Solution (HBSS) were purchased from HyClone Co., Ltd. (Utah, USA). Fetal bovine serum (FBS) was purchased from gibco Co., Ltd. (New York, USA). PMSF and RIPA buffer were purchased from Thermo Fisher Scienti c Inc. (Illinois, USA). The puri ed water used in this study was prepared using a Mille-Q system (EMD Millipore, Billerica, MA, USA). HPLC-grade acetonitrile and methanol were obtained from Thermo Fisher Scienti c (Waltham, MA, USA). All other reagents were analytical grade.
+ Open protocol
+ Expand
3

Optimizing Drug Delivery Formulations

Check if the same lab product or an alternative is used in the 5 most similar protocols
CsA was purchased from Taishang Chemical Pharmacy Company (Taishang, China). Cyclosporin D (CsD) was purchased from Shanghai Tongtian Biotechnology Company (Shanghai, China). Vitamin E polyethylene glycol succinate (TPGS) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Sodium dodecyl sulfate (SDS) was purchased from BASF, Ludwigshafen, Germany. Hydroxypropyl cellulose (HPC) was purchased from Nippon Soda Co., Ltd. (Tokyo, Japan). Roswell Park Memorial Institute (RPMI) 1640 medium, phosphate buffered saline (PBS) and Hank’s Balanced Salt Solution (HBSS) were purchased from HyClone Co., Ltd. (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from gibco Co., Ltd. (GrandIsland, NY, USA). PMSF and RIPA buffer were purchased from Thermo Fisher Scientific Inc. (Rochester, NY, USA). The purified water used in this study was prepared using a Mille-Q system (EMD Millipore, Billerica, MA, USA). HPLC-grade acetonitrile and methanol were obtained from Thermo Fisher Scientific (Waltham, MA, USA). All other reagents were analytical grade.
+ Open protocol
+ Expand
4

Exploring Cyclosporin Formulations and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cyclosporin A (CsA) was purchased from Taishang Chemical Pharmacy Company (Taishang, China). Cyclosporin D (CsD) was purchased from Shanghai Tongtian Biotechnology Company (Shanghai, China). Neoral® (Cyclosporine soft gelatin capsules) was purchased from a local drugstore. Vitamin E polyethylene glycol succinate (TPGS) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Sodium dodecyl sulfate (SDS) was purchased from BASF, Ludwigshafen, Germany. Hydroxypropyl cellulose (HPC) was purchased from Nippon Soda Co., Ltd. (Tokyo, Japan). Simulated intestinal fluid (SIF) and simulated gastric fluid (SGF) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The purified water used in this study was prepared using a Milli-Q system (EMD Millipore, Billerica, MA, USA). HPLC-grade acetonitrile and methanol were obtained from Thermo Fisher Scientific (Waltham, MA, USA). All the other reagents were of analytical grade.
+ Open protocol
+ Expand
5

Bioavailability Enhancement of Itraconazole

Check if the same lab product or an alternative is used in the 5 most similar protocols
ITZ of pharmaceutical grade was purchased from Sanlian Pharmaceutical (Harbin, China), its active metabolite hydroxyitraconazole (OH-ITZ) (purity higher than 99%) was purchased from J&K Scientific Ltd. (Shanghai, China) and used as a reference substance. Commercial Sporanox® capsules were obtained from Xian Janssen Pharmaceutical Ltd. (Xian, China). d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS), Poloxamer 188, and sodium dodecyl sulfate (SDS) were purchased from BASF (Ludwigshafen, Germany), Macklin Biochemical Ltd. (Shanghai, China), and FuchenChemical Reagents Factory (Tianjin, China), respectively. Hydroxypropylmethylcellulose acetate succinate (HPMC AS) (grade LF) was kindly provided by Shin Etsu (Shin-Etsu Chemical Co., Ltd., Tokyo, Japan). The beagle dogs used for in vivo bioavailability study were purchased from the ChaiMen Biological Technology Ltd. (Nanjing, China). HCl was obtained from Jinkun chemical co., Ltd (Bengbu, China), the Na3PO4, NaH2PO4 and Na2HPO4 were purchased from Damao Chemical Reagent Factory (Tianjin, China). All reagents were of grade analytical and used without further purification.
+ Open protocol
+ Expand
6

Solubilization and Characterization of Various Pharmaceutical Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phenytoin (PHT) was purchased from Shizuoka Caffeine Industries Co., Ltd. (Shizuoka, Japan). Mefenamic acid (MFA) was purchased from Cheng Fong Chemical Co., Ltd. (Tu Cheng, Taipei). Itraconazole (ITZ) was purchased from J&H Chemical Co., Ltd. (Hangzhou, China). FNB was purchased from Combi-Blocks Inc. (San Diego, CA, USA). Sulfamethoxazole (SMX) was purchased from Virchow Laboratories Ltd. (Telangana, India). Table 1 shows the representative physicochemical properties of these drugs. Polyvinylpyrrolidone (PVP) K-25 and sodium dodecyl sulfate (SDS) were purchased from BASF Japan Ltd. (Tokyo, Japan). Yttria-stabilized zirconia beads (0.3-mm diameter) were purchased from Nikkato Corp. (Osaka, Japan). All other chemicals and solvents were of analytical reagent grade, and purified water was used for the solution preparation.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!