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Mouse il 21r fc

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Mouse IL-21R–Fc is a recombinant protein that consists of the extracellular domain of the mouse interleukin-21 receptor (IL-21R) fused to the Fc region of human IgG1. It is produced in mouse myeloma cell line NS0 cells.

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Lab products found in correlation

Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Isotype matched control, by R&D Systems (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Hepes buffer, by Corning (1 mentions) Anti granzyme b pe, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Mouse IL-21R-human Fc (2 citations) Recombinant mouse IL-21R Fc Chimera protein (2 citations) Recombinant mouse IL-21R human FC chimera (2 citations)

2 protocols using mouse il 21r fc

1

Alloantibody Production Assay

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Sorted cTFH were cocultured with sorted autologous RM, AM, or TLM cells (2 × 104) at 1:1 ratio with SEB (1 μg/mL, Toxin Technology) in RPMI (Gibco, Thermo Fisher Scientific) supplemented with 10% FCS, 100 IU/mL penicillin, 100 mg/mL streptomycin (Life Technologies, Thermo Fisher Scientific), 1 M HEPES buffer (Corning), and 2 mM l-glutamine. Some cocultures were supplemented with mouse IL-21R–Fc (R&D Systems, Bio-Techne) or isotype-matched control (R&D Systems, Bio-Techne). After 6 days of coculture, cells were stained with CD4, CD19, CD27, CD38, and CD71 antibodies before acquisition on the cytometer. DSAs were detected in supernatants after 6 days of coculture using Luminex SAB assay.
Louis K., Bailly E., Macedo C., Lau L., Ramaswami B., Chang A., Chandran U., Landsittel D., Gu X., Chalasani G., Zeevi A., Randhawa P., Singh H., Lefaucheur C, & Metes D. (2021). T-bet+CD27+CD21– B cells poised for plasma cell differentiation during antibody-mediated rejection of kidney transplants. JCI Insight, 6(12), e148881.
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2

Flow Cytometric Analysis of Lymphocytes

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Lymphocytes harvested from spleen and other organs were stained and analyzed by flow cytometry. All antibodies were purchased from eBiosciences (San Diego, CA) and BD Biosciences (San Jose, CA), except anti-Granzyme B PE (Invitrogen, NY), BATF (Cell signaling, MA) and Mouse IL-21 R Fc (R&D Systems, MN). Intracellular staining of transcriptional factors and BATF were performed with Foxp3 nuclear staining protocol (eBioscience). All flow cytometry data were acquired on an LSRII (BD Biosciences) and analyzed by FlowJo (Treestar).
Xin G., Schauder D., Lainez B., Weinstein J., Dai Z., Chen Y., Esplugues E., Wen R., Wang D., Parish I., Zajac A.J., Craft J, & Cui W. (2015). A Critical Role of IL-21 Induced BATF in Sustaining CD8 T Cell Mediated Chronic Viral Control. Cell reports, 13(6), 1118-1124.
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