Dnase type 1

Manufactured by Worthington

Sourced in Germany, United States

DNase Type I is a laboratory enzyme used for the controlled degradation of DNA. It catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, resulting in the breakdown of DNA molecules. The enzyme is commonly used in various biological and molecular biology applications that require the removal or manipulation of DNA.

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Lab products found in correlation

Neutral protease, by Worthington (1 mentions) Type 1 collagen from rat tail, by BD (1 mentions) M csf, by R&D Systems (1 mentions) Collagenase type 1, by Worthington (1 mentions) Rapamycin, by InvivoGen (1 mentions) Carbamazepine, by Bio-Techne (1 mentions) Bafilomycin a1, by InvivoGen (1 mentions) Everolimus, by InvivoGen (1 mentions) Goat anti mouse igg1 af488, by Thermo Fisher Scientific (1 mentions) Anti human lc3 4e12, by MBL Life Science (1 mentions) Dispase 2, by Roche (1 mentions) Collagenase d, by Roche (1 mentions)

Close spelling variants of this product

DNase I Type I DNase

3 protocols using dnase type 1

Collagen-Fibrin Scaffold Characterization

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The following reagents were purchased from commercial sources: Collagen type I from rat tail (BD Biosciences, Franklin Lakes, NJ), Tissue-Tek® Biopsy Cryomold® (10 mm × 10 mm × 5 mm, Sakura Finetek USA, Inc.), plasminogen-depleted, FXIII-containing fibrinogen from human plasma and citrate-free thrombin from human plasma (Merck Millipore, Darmstadt, Germany), AF647 labeling kit, AF488-conjugated 10 kDa dextran and AF647-conjugated human fibrinogen (Life Technologies, Grand Island, NY), Collagenase Type I, DNase Type I, and Neutral Protease (Worthington Biochemical, Lakewood, NJ), Rat-anti-Mouse MR/CD206 monoclonal antibody (clone MR5D3, Bio-Rad, Hercules, CA), Rabbit-anti-Rat secondary HRP-conjugated antibody (Dako, Glostrup, Denmark). Recombinant IL-4, IL-13, M-CSF, and GM-CSF (R&D systems, Minneapolis, MN). Monoclonal mouse anti-uPARAP antibody 2h9 was described previously [61 (link)]. Reagents and antibodies used for flow cytometry are described in the flow cytometry section below.

Jürgensen H.J., Silva L.M., Krigslund O., van Putten S., Madsen D.H., Behrendt N., Engelholm L.H, & Bugge T.H. (2019). CCL2/MCP-1 signaling drives extracellular matrix turnover by diverse macrophage subsets. Matrix Biology Plus, 1, 100003.

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Isolation of Mouse Renal Inner Medulla Cells

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Mouse IMCD were isolated as previously described (Strait et al. 2010 (link)). Briefly, mouse renal inner medullas were minced and incubated at 37 °C in 0.1 % collagenase (type I; Worthington, Freehold, NJ, USA) containing 0.01 % DNase (type I) in Hanks’ balanced salt solution (HBSS) +15 mM HEPES (pH 7.4). After ~45 min, the digest containing mainly single cells and tubules was filtered through a 74-µm mesh, centrifuged, and suspended in 10 % bovine serum albumin in HBSS, followed by two centrifuge/washes with HBSS. The final pellet containing primarily tubules was suspended in HBSS + HEPES.

RAMKUMAR N., STUART D., ABRAHAM N, & KOHAN D.E. (2018). Nephron Prorenin Receptor Deficiency Alters Renal Medullary Endothelin-1 and Endothelin Receptor Expression. Physiological research, 67(SUPPLEMENTUM 1), S127-S136.

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Multiparametric Flow Cytometry Analysis

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The following antibodies were used: anti-human CD1a (HI149-APC; BD Pharmigen), CD3 (UCTH1-APC-FIRE750; Biolegend), anti-human CD8a (HIT8a-PerCP-Cy5.5), anti-human CD14 (HCD14-PE-Dazzle594), anti-human CD11c (B-ly6-PE-Cy7), anti-HIV-1 capsid protein p24 (KC57-PE; Beckman Coulter), anti-human LC3 (4E12; MBL Life Science), goat anti-mouse IgG1 (AF488; Invitrogen A-21121).
The following reagents were used: bafilomycin A1 (Invivogen), carbamazepine (Tocris), everolimus (Invivogen), rapamycin (Invivogen), Zidovudine (AZT; NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH), paraformaldehyde (8%, Electron Microscopy Sciences, Aurion), dispase II (Roche Diagnostics), ethylenediaminetetraacetic acid (EDTA; Sigma-Aldrich), 2 mM 1,4-dithiothreitol (DTT; Sigma-Aldrich), Collagenase D (Roche), DNAse type I (Worthington Biochemical Corporation).

Cloherty A.P., van Teijlingen N.H., Eisden T.J., van Hamme J.L., Rader A.G., Geijtenbeek T.B., Schreurs R.R, & Ribeiro C.M. (2021). Autophagy-enhancing drugs limit mucosal HIV-1 acquisition and suppress viral replication ex vivo. Scientific Reports, 11, 4767.

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