Fitc conjugated anti rabbit igg solution

The procedure for immobilization of bioreceptor and target is similar to the one described in section 4.4.1. Four diazonium modified SiCN chips (A2, B2, C2, D2) were incubated in 100 µg/mL goat anti-rabbit IgG solution (polyclonal, Sigma-Aldrich) for 2 h, and 5% BSA (‎bovine serum albumin) for 1 h at room temperature. Chips A2 and B2 were incubated in 200 µg/mL rabbit IgG solution (polyclonal, Sigma-Aldrich) for 1 h whereas chips C2 and D2 were subject to 200 µg/mL goat IgG solution (polyclonal, Sigma-Aldrich) as control for 1 h at room temperature. Chips A2 and C2 were immersed in 1:200 diluted FITC conjugated anti-rabbit IgG solution (Sigma-Aldrich) while Chips B2 and D2 were immersed in 1:400 diluted FITC conjugated anti-rabbit IgG solution at room temperature for 1 h.