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Dylight 488 labeled lycopersicon esculentum tomato lectin

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DyLight 488 Labeled Lycopersicon esculentum (Tomato) Lectin is a fluorescently labeled lectin derived from the tomato plant. It is used as a tool for the detection and localization of carbohydrate-containing structures in biological samples.

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Lab products found in correlation

Fitc dextran, by Leica (1 mentions) Tcs sp5 2 dichroic cs confocal microscope, by Leica (1 mentions) Fitc dextran, by Merck Group (1 mentions) Buffered formalin, by Thermo Fisher Scientific (1 mentions) A1 confocal microscope, by Nikon (1 mentions)

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DyLight 488 (29 citations) Lycopersicon esculentum (tomato) lectin (24 citations) Tomato lectin (21 citations) Lycopersicon esculentum lectin (19 citations) Lycopersicon esculentum (16 citations) DyLight-488 Tomato Lectin (6 citations) DyLight 488 Lycopersicon Esculentum Lectin (5 citations) DyLight 488 labeled tomato lectin (4 citations) DyLight 488-labeled Lycopersicon esculentum lectin (2 citations) DyLight 488–labeled lectin (2 citations)

4 protocols using dylight 488 labeled lycopersicon esculentum tomato lectin

1

Vascular Perfusion and Leakage Assay

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On the day of the last PCN injection, a vascular perfusion assay was performed by injecting 100 μl of DyLight® 488-labeled Lycopersicon esculentum (tomato) lectin (1 mg/ml, Vector Lab) intravenously 30 min prior to sacrifice. To check for vascular leakage, 100 μl of FITC-dextran (25 mg/ml, 70 kDa, Sigma-Aldrich) was intravenously injected into the mice 30 min before sacrifice. After the mice were perfused with PBS and 4% paraformaldehyde (PFA), the FITC-dextran or tomato-lectin in the tumor tissues were observed under a Leica TCS SP5 II Dichroic/CS confocal microscope (Leica, Wetzlar, Germany). The fluorescence intensity was measured using ImageJ software.
Choi Y.S., Jang H., Gupta B., Jeong J.H., Ge Y., Yong C.S., Kim J.O., Bae J.S., Song I.S., Kim I.S, & Lee Y.M. (2020). Tie2-mediated vascular remodeling by ferritin-based protein C nanoparticles confers antitumor and anti-metastatic activities. Journal of Hematology & Oncology, 13, 123.
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2

In Vivo Cerebral Vessel Staining

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To visualize cerebral blood vessels for histological analysis, mice received an in vivo injection of 100 μl fluorescein-labeled Lectin [DyLight 488 Labeled Lycopersicon Esculentum (Tomato) Lectin, DL-1174, Vector Laboratories, Burlingame, CA] though the femoral vein to stain endothelial cells of perfused blood vessels. Next, the animals were transcardially perfused with 4% paraformaldehyde in phosphate buffered saline and the isolated brains were stored in the same solution at 4°C for 72 h and then kept in 0.1% sodium azide in phosphate buffered saline.
de Bortoli T., Boehm-Sturm P., Koch S.P., Nieminen-Kelhä M., Wessels L., Mueller S., Ielacqua G.D., Klohs J., Vajkoczy P, & Hecht N. (2021). Three-Dimensional Iron Oxide Nanoparticle-Based Contrast-Enhanced Magnetic Resonance Imaging for Characterization of Cerebral Arteriogenesis in the Mouse Neocortex. Frontiers in Neuroscience, 15, 756577.
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3

Visualizing Nanoparticle Uptake in Microglia

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The BV-2 cells were ready for confocal imaging study after 1–2-day culturing on 35 mm imaging dish. First, 50 μL of QD-bEVs was mixed with 1950 μL of cell culture media and added to 1 dish of BV-2 cells. Then 50 μL of pQDs (f7-f9 collected from qEV column purified QD-4FB) was mixed with 1950 μL of cell culture media and added to 1 dish of BV-2 cells, as the negative control. BV-2 cells were incubated at 37 °C in a 5% CO2 atmosphere for 24 h, fixed with 10% buffered formalin (ThermoFisher) for 10 min, and then washed twice with 1×PBS. The cells were then stained with DyLight 488 Labeled Lycopersicon esculentum (Tomato) Lectin (Vector Laboratories) at 1:1000 ratio in 1×PBS for 1 h. Cells were then imaged by a Nikon A1 confocal microscope (Nikon) under same camera and laser setting. Z-stack images at 60× or 4-time zoom in at 60× were taken, and maximum-intensity projections were formed.
Zhang M., Vojtech L., Ye Z., Hladik F, & Nance E. (2020). Quantum Dot Labeling and Visualization of Extracellular Vesicles. ACS applied nano materials, 3(7), 7211-7222.
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4

Visualizing Cellular Uptake of Quantum Dot-labeled Extracellular Vesicles

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After 1–2 days of BV-2 cell culturing on 35 mm imaging dish, the cells were stained with DyLight 488 Labeled Lycopersicon esculentum (Tomato) Lectin (Vector Laboratories) at 1:1000 ratio in prewarmed cell culture media at 37 °C in a 5% CO2 atmosphere for 1 h. The cells were then washed twice with cell culture media. Then 35 μL of QD-bEVs was mixed with 950 μL of cell culture media and added to 1 dish of BV-2 cells. After 1 and 4 h incubation at 37 °C in a 5% CO2 atmosphere, the 35 mm dish of BV-2 cells was quickly moved to an incubation chamber (37 °C, 5% CO2, and over 40% humidity) to perform time-lapse imaging. Images were taken at 60× every 5 min for 1 h at various locations in the chamber.
Zhang M., Vojtech L., Ye Z., Hladik F, & Nance E. (2020). Quantum Dot Labeling and Visualization of Extracellular Vesicles. ACS applied nano materials, 3(7), 7211-7222.
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