Splenocyte T and B cell ELISPOT reagents were obtained from Mabtech (Mariemont, OH). H1HA specific T cells were quantified per manufactures instructions. Positive controls were stimulated with 20 ng/ml PMA (Sigma-Aldrich, St. Louis, MO) and 500 ng/ml ionomycin (Sigma-Aldrich). The H1HA relevant peptide used was a CD8+ cell-restricted HA peptide (IYSTVASSL). H1HA-specific antibody secreting cells were detected by coating of MAIPSWU (Millipore, Billerica, MA, USA) plates with the transiently expressed H1HA antigen utilized for ELISA (~1.0 μg/well). Positive spots were visualized on a CTL Imager and counting was performed with Immunospot software (Cellular Technology Ltd., Shaker Heights, OH)
Maipswu
Manufactured by Merck Group
Sourced in United States
The MAIPSWU is a laboratory equipment product designed for specific functions. It is a tool used in scientific research and experiments. The core function of the MAIPSWU is to facilitate various laboratory procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
2 protocols using maipswu
1
Quantifying H1HA-Specific T and B Cells
2
Quantification of H1HA-specific T and B Cells
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