For stem-cell quantitation, the cultured bone marrow nucleated cells were co-stained with a combination of PE-Cy7 labeled antibodies against mouse CD3, CD4, CD8, B220, Gr-1, Mac-1 and TER-119, anti-Sca-1-PercpCy5.5, anti-CD48-PE and anti-CD150-APC (all from Biolegend). DAPI was used for dead-cell discrimination. Due to the presence of cytokine stem cell factor (SCF), we could not detect the c-Kit marker of those cultured cells. So hereafter we did not use c-Kit in detecting subpopulation. Flow cytometry and flow sorting were conducted with a CyAn (DakoCytomation) or a FACSCalibur system (BD Biosciences). FlowJo software Version 7.6.2 (TreeStar) was used for data analysis.
Anti sca 1 percpcy5
Manufactured by BioLegend
Sourced in United States
Anti-Sca-1-PercpCy5.5 is a fluorescently-labeled antibody that binds to the Sca-1 (Stem Cell Antigen-1) protein. Sca-1 is a cell surface marker commonly used to identify and characterize stem and progenitor cells in various tissues. The PerCP-Cy5.5 fluorescent dye is conjugated to the antibody, allowing for detection and analysis of Sca-1-positive cells using flow cytometry.
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Lab products found in correlation
Anti cd150 apc, by BioLegend (1 mentions)
Facscalibur system, by BD (1 mentions)
Anti pdgfrα cd140a apc, by BioLegend (1 mentions)
Anti cd206 percp cy5, by BioLegend (1 mentions)
Anti f4 80 apc, by BioLegend (1 mentions)
Anti cd34 pe, by BioLegend (1 mentions)
Anti cd11c fitc, by BD (1 mentions)
Facsverse, by BD (1 mentions)
2 protocols using anti sca 1 percpcy5
1
Stem Cell Quantification Protocol
2
Flow Cytometric Analysis of Stromal and Immune Cells
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The SV fraction suspended in PBS containing 2% fetal calf serum was incubated with a
mixture of antibodies containing either anti-CD31-PE-Cy (BioLegend, San Diego, CA,
U.S.A.), anti-CD34-PE (BioLegend), anti-Sca1-PerCP/Cy5.5 (BioLegend), and anti-PDGFRα
(CD140a)-APC (BioLegend) or containing anti-CD11c-FITC (BD Biosciences, San Jose, CA,
U.S.A.), anti-CD206-PerCP/Cy5.5 (BioLegend), and anti-F4/80-APC (BioLegend) for 30 min on
ice. After centrifugated at 1,000 × g for 10 min at 4°C, the supernatant
was discarded, and the pellet was suspended in PBS containing 2% FCS. The suspension was
filtered through a 40 µm nylon filter and analyzed on a flow cytometer
(BD FACSVerse, BD Biosciences) with singlet discrimination to detect APC, PE-Cy, PE, and
PerCP/Cy5.5 stained cells.
mixture of antibodies containing either anti-CD31-PE-Cy (BioLegend, San Diego, CA,
U.S.A.), anti-CD34-PE (BioLegend), anti-Sca1-PerCP/Cy5.5 (BioLegend), and anti-PDGFRα
(CD140a)-APC (BioLegend) or containing anti-CD11c-FITC (BD Biosciences, San Jose, CA,
U.S.A.), anti-CD206-PerCP/Cy5.5 (BioLegend), and anti-F4/80-APC (BioLegend) for 30 min on
ice. After centrifugated at 1,000 × g for 10 min at 4°C, the supernatant
was discarded, and the pellet was suspended in PBS containing 2% FCS. The suspension was
filtered through a 40 µm nylon filter and analyzed on a flow cytometer
(BD FACSVerse, BD Biosciences) with singlet discrimination to detect APC, PE-Cy, PE, and
PerCP/Cy5.5 stained cells.
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