The detailed protocol for western blotting has been described previously44 (link). The primary antibodies were as follows: anti-E-cadherin, N-cadherin, EGFR, EGFR-19del (exon19 E746-A750del specific), vimentin (R28), LIN28B (Cell Signaling Technology, Beverly, MA), and ZEB1 (H-102, Santa Cruz Biotechnology, Santa Cruz, CA). Monoclonal anti-actin antibody, used as an equal loading control, was purchased from Merck Millipore (Billerica, MA). The following secondary antibodies were used: goat anti-rabbit or anti-mouse immunoglobulin G (IgG)-conjugated horseradish peroxidase (Santa Cruz Biotechnology). To detect specific signals, the membranes were examined using ECL Prime Western Blotting Detection System (GE Healthcare, Amersham, UK).
Anti mouse immunoglobulin g igg conjugated horseradish peroxidase
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-mouse immunoglobulin G (IgG)-conjugated horseradish peroxidase is a laboratory reagent that consists of horseradish peroxidase enzyme covalently linked to anti-mouse IgG antibodies. This product can be used to detect and quantify mouse IgG proteins in various immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
Ecl prime western blotting detection system, by GE Healthcare (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Anti actin monoclonal antibody, by Merck Group (1 mentions)
Lin28b, by Cell Signaling Technology (1 mentions)
Zeb1 h 102, by Santa Cruz Biotechnology (1 mentions)
Vimentin r28, by Cell Signaling Technology (1 mentions)
N cadherin, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Specific antibodies against β actin, by Santa Cruz Biotechnology (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt reagent, by Merck Group (1 mentions)
P erk, by Santa Cruz Biotechnology (1 mentions)
P jnk, by Santa Cruz Biotechnology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
2 protocols using anti mouse immunoglobulin g igg conjugated horseradish peroxidase
1
Comprehensive Western Blotting Protocol
2
Anti-inflammatory Protein Detection Assay
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Specific antibodies against β-actin, iNOS, COX-2, NF-κB p65, p-p38, p-ERK, p-JNK, and anti-mouse immunoglobulin G (IgG)conjugated horseradish peroxidase were obtained from Santa Cruz (USA). LPS, dimethylsulfoxide (DMSO), and 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagents were purchased from Sigma-Aldrich Co., LLC. (USA). Enzyme-linked immunosorbent assay (ELISA) kits for TNF-α, IL-6, and IL-1β were purchased from BD Biosciences (USA) and Dulbecco's Modified Eagle's Medium (DMEM) from GIBCO (USA). BCA protein assay kit and enhanced chemiluminescence kit (ECL kit) were from Pierce (USA), and fetal bovine serum (FBS) and penicillin/streptomycin were purchased from Hyclone (USA).
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