4T1 exosomes were labelled with PKH67 green fluorescent (PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labelling, #MIDI67, Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer's instructions. Briefly, exosome pellets were labelled with 2.5 μM of PKH67 dye in 400 μl of diluent C for 5 minutes. Afterwards, blocking buffer (1% of bovine serum albumin) was added and incubated for 1 minute. Finally, exosomes were washed with PBS by ultracentrifugation at 120,000g, for 2 hours, 4°C. PKH67-labeled exosomes were then resuspended in 150μl PBS.
Pkh67 green fluorescent cell linker midi kit for general cell membrane labelling
Manufactured by Merck Group
Sourced in United States
The PKH67 Green Fluorescent Cell Linker Midi Kit is a laboratory product designed for general cell membrane labeling. It utilizes a fluorescent dye that binds to cell membranes, allowing for the visualization and tracking of cells.
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2 protocols using pkh67 green fluorescent cell linker midi kit for general cell membrane labelling
1
Fluorescent Labeling of 4T1 Exosomes
2
EV Membrane Labeling Protocol
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EVs were labelled with PKH67 green fluorescent dye according to the manufacturer's instructions (PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labelling, Sigma-Aldrich). Briefly, EVs were labelled with 2.5 µM of PKH67 dye in 400 µl of diluent C for 5 min, and then blocked for 1 min in 1% bovine serum albumin (BSA), after which EVs were washed with a high volume of PBS by ultracentrifugation for 2 h at 120,000g and at 4°C. PKH67-labelled EVs were then resuspended in PBS and stored at −80°C.
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