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Anti phospho ulk ser555

Manufactured by Cell Signaling Technology
Sourced in United States

Anti-phospho-ULK Ser555 is a primary antibody that specifically recognizes the ULK1 protein phosphorylated at serine 555. ULK1 is a serine/threonine protein kinase that plays a key role in the initiation of autophagy.

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2 protocols using anti phospho ulk ser555

1

Antibody Sourcing for Western Blot and Immunostaining

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Anti-beta actin-HRP, catalog number 5125S, and anti-phospho-ULK Ser555, catalog number 5869, were purchased from Cell Signaling Technology (Danvers, MA). Anti-DAT (for western blot analysis), anti-N-terminus, catalog number sc-32258, anti-ULK1 antibody, catalog number sc-33182, and anti-EGFR, catalog number: sc-03-G, were purchased from Santa Cruz Biotechnology (Paso Robles, CA). Anti-serotonin transporter (SERT), catalog number PA1706, was purchased from Boster Bio (Pleasanton, CA). Anti-tyrosine hydroxylase (TH), catalog number 818001, was purchased from BioLegend (San Diego, CA). Anti-DAT (anti-C-terminus), catalog number AB1766, and anti-DAT for immunostaining, catalog number MAB369, were purchased from Millipore Sigma (Burlington, MA). Anti-VPS34, catalog number 3811S, and anti-phospho-VPS34 Ser249, catalog number 13857S, for western blot were purchased from Cell Signaling Technology (Danvers, MA). LAMP2A antibody was purchased from (Thermo Fisher Scientific, USA). GFP antibody, catalog number ab13970 was pruchased from Abcam (USA).
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2

Antibody-based Protein Analysis in Autophagy

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Antibodies against microtubule-associated protein light chain 3 (LC3) and α-tubulin were obtained from Sigma Chemical Co. (St Louis, MO, USA) and Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA), respectively. Anti-p21Waf1/Cip1, anti-p27Kip1, anti-cyclin D1, anti-caspase-3, poly (ADP-ribose) polymerase (PARP), anti-c-jun-N-terminal kinase (JNK), anti-phospho-JNK (Thr183/Tyr185), anti-adenosine monophosphate-activated protein kinase (AMPK), anti-phospho-AMPK (Thr172), anti-ULK, anti-phospho-ULK (Ser555), anti-c-jun, anti-phospho-c-jun (Ser73), anti-ERK1/2, anti-phospho-ERK1/2 (Thr202)/Tyr204), anti-p38, anti-phospho-p38 (Thr180/Tyr182), anti-p53, anti-phospho-p53 (Ser15), anti-Bcl-2, anti-p62/SQSTM1, and anti-Beclin-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Propidium iodide (PI), Ribonuclease A (RNase A) from bovine pancreas, monodansyl cadaverine (MDC), 2′7′-dichlorofulorescein diacetate (DCF-DA), Bafilomycin A1 from Streptomyces griseus, and 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma Chemical. SP600125, SB203580, PD98059, and N-acetyl-L-cysteine (NAC) were obtained from Calbiochem (San Diego, CA).
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