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Anicomycin

Manufactured by Merck Group
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Anicomycin is a laboratory reagent manufactured by Merck Group. It is a chemical compound used for research and analysis purposes in various scientific applications.

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Lab products found in correlation

Optiprep, by Merck Group (2 mentions) Triptolide, by Merck Group (2 mentions) Actinomycin d, by Merck Group (2 mentions)

2 protocols using anicomycin

1

Transcardial Perfusion and Tissue Dissociation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Once anesthetized the animals were transcardially perfused with ice-cold Choline solution (above) containing the following small molecule cocktail for 5 minutes: 1 uM TTX (Sigma), 100 uM AP-V (Thermo Fisher Scientific), 5 ug/ml Actinomycin D (Sigma) and 10 uM Triptolide (Sigma). V1 was then microdissected, cut into 300um slices, and incubated on ice for 30 minutes in Dissociation Solution containing 1uM TTX, 100 uM AP-V (Thermo Fisher Scientific), 5 ug/ml Actinomycin D (Sigma), 10 uM Triptolide (Sigma) and 10 ug/ml Anicomycin (Sigma). Papain was added to a final concentration of 20 U/ml and the tissue dissociated for 1h at 37°C with gentle agitation in a total volume of 3.2ml. The remaining procedures were performed per manufacturer’s instructions without the small molecule cocktail. Following gradient centrifugation the cells were washed in Dissociation Solution containing 0.04% BSA and resuspended in Dissociation Solution containing 0.04% BSA and 15% Optiprep (Sigma) for single cell RNA-Seq.
Hrvatin S., Hochbaum D.R., Nagy M.A., Cicconet M., Robertson K., Cheadle L., Zilionis R., Ratner A., Borges-Monroy R., Klein A.M., Sabatini B.L, & Greenberg M.E. (2017). Single-Cell Analysis of Experience-Dependent Transcriptomic States in Mouse Visual Cortex. Nature neuroscience, 21(1), 120-129.
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2

Transcardial Perfusion and Tissue Dissociation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Once anesthetized the animals were transcardially perfused with ice-cold Choline solution (above) containing the following small molecule cocktail for 5 minutes: 1 uM TTX (Sigma), 100 uM AP-V (Thermo Fisher Scientific), 5 ug/ml Actinomycin D (Sigma) and 10 uM Triptolide (Sigma). V1 was then microdissected, cut into 300um slices, and incubated on ice for 30 minutes in Dissociation Solution containing 1uM TTX, 100 uM AP-V (Thermo Fisher Scientific), 5 ug/ml Actinomycin D (Sigma), 10 uM Triptolide (Sigma) and 10 ug/ml Anicomycin (Sigma). Papain was added to a final concentration of 20 U/ml and the tissue dissociated for 1h at 37°C with gentle agitation in a total volume of 3.2ml. The remaining procedures were performed per manufacturer’s instructions without the small molecule cocktail. Following gradient centrifugation the cells were washed in Dissociation Solution containing 0.04% BSA and resuspended in Dissociation Solution containing 0.04% BSA and 15% Optiprep (Sigma) for single cell RNA-Seq.
Hrvatin S., Hochbaum D.R., Nagy M.A., Cicconet M., Robertson K., Cheadle L., Zilionis R., Ratner A., Borges-Monroy R., Klein A.M., Sabatini B.L, & Greenberg M.E. (2017). Single-Cell Analysis of Experience-Dependent Transcriptomic States in Mouse Visual Cortex. Nature neuroscience, 21(1), 120-129.
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