Abi 3730xl sanger sequencing device

Manufactured by Thermo Fisher Scientific

Sourced in United States

The ABI 3730XL is a Sanger sequencing device manufactured by Thermo Fisher Scientific. It is designed for high-throughput DNA sequencing, with the ability to generate up to 96 sequencing reactions per run. The instrument utilizes capillary electrophoresis technology to separate and detect fluorescently labeled DNA fragments, providing accurate and reliable DNA sequence data.

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Lab products found in correlation

Bigdye terminator v3.1 cycle sequencing kit, by Thermo Fisher Scientific (4 mentions) Exosap ittm pcr product cleanup reagent, by Thermo Fisher Scientific (2 mentions)

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ABI 3730XL (295 citations) Sanger sequencing (66 citations)

4 protocols using abi 3730xl sanger sequencing device

Sequencing and Phylogenetic Analysis of Xylem Nematode

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Sequence alignments of one of the P. vulnus population from Cihanbeyli district in Konya province (sample number: 184) were determined.
Nematode DNAs was purified using ExoSAP-ITTM PCR Product Cleanup Reagent (Thermo Fisher Scientific, USA) prior to sequence alignment.
Purified DNA samples were sequenced using ABI3730XL Sanger sequencing device and BigDye Terminator v3.1 Cycle sequencing kit (Applied Biosystems, Foster City, CA) in Macrogen laboratory in the Netherlands.
The sequences were deposited into the GenBank database and compared with those of the other P. vulnus populations available at the GenBank sequence database using the BLAST homology search tool (https://blast.ncbi.nlm.nih.gov/Blast.cgi).

Karaca M.S., Yavuzaslanoglu E., Imriz G, & Sonmezoglu O.A. (2020). Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey. Journal of Nematology, 52, e2020-84.

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Mutagenesis and Purification of CatPO

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Oligonucleotides (Table 1 ▸) with the desired mutations (E484A, E484D, E484I, T188A, T188D, T188F and T188I) were obtained from Sentegene and used to mutate the catpo gene in the pET-28-TEV-CATPO plasmid according to the procedure described previously (Yuzugullu, Trinh, Smith et al., 2013 ▸ ). Mutations were confirmed by DNA sequencing (MedSantek, Türkiye) on an ABI 3730XL Sanger sequencing device (Applied Biosystems, USA) using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, USA). Thereafter, sequential expression and purification were performed as described previously (Yuzugullu, Trinh, Smith et al., 2013 ▸ ; Yuzugullu, Trinh, Fairhurst et al., 2013 ▸ ).

Yuzugullu Karakus Y., Goc G., Zengin Karatas M., Balci Unver S., Yorke B.A, & Pearson A.R. (2024). Investigation of how gate residues in the main channel affect the catalytic activity of Scytalidium thermophilum catalase. Acta Crystallographica. Section D, Structural Biology, 80(Pt 2), 101-112.

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COI Gene Sequence Analysis

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PCR products were purified using the procedures of ExoSAP-ITTM PCR Product Cleanup Reagent (Thermo Fisher Scientific, USA) kit by BM Labosis. The purified PCR products were mixed with forward and reverse primers of the COI gene and sequence analysis was performed. Sequence analysis was carried out in the Macrogen Netherlands laboratory by using the ABI 3730XL Sanger sequencing device (Applied Biosystems, Foster City, CA) and BigDye Terminator v3.1 Cycle Sequencing Kit.

Polat F., & Öcal İ.Ç. (2021). Phylogenetic analysis of Euscorpius phrygius Bonacina, 1980 (Scorpiones: Euscorpiidae) distributed in Turkey. Biological Diversity and Conservation.

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Molecular Phylogenetic Analysis of EPF Isolates

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The genomic DNA of the tested EPF isolates was extracted through CTAB method described by Doyle and Doyle (1990) . The PCR (polymerase chain reaction) was conducted in a gradient thermal cycler, using two primers, based on ITS-rDNA region gene sequences, ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4 (5'-TCCTCCGCTTATTGATATGC-3') (White et al., 1990) .
The PCR products were sequenced with the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, CA) and the ABI 3730XL Sanger sequencing device (Applied Biosystems, CA) in the Macrogen laboratory in Netherlands. The DNA sequences of EPF isolates were performed using the Bioedit program with ClustalW algorithm (Thompson et al., 1994; Hall, 1999) . Molecular phylogenetic analyses were executed through the maximum likelihood (ML) method based on the Tamura 3-parameter model, using MEGA7 software (Biodesign Institute, Arizona) (Kimura, 1980; Tamura et al., 2011) . The phylogenetic analysis was performed using the ITS region sequence of EPF isolates and the nucleotide sequence of the other isolates of the respective species retrieved from GenBank (Altschul et al., 1997) .

Baki D., Tosun H.Ş., & Erler F. (2021). Indigenous entomopathogenic fungi as potential biological control agents of rose sawfly, Arge rosae L. (Hymenoptera: Argidae). TURKISH JOURNAL OF ZOOLOGY, 45(7), 517-525.

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