Mouse anti human cd14 pe cy5

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

Mouse anti-human CD14-PE-Cy5.5 is a fluorochrome-conjugated monoclonal antibody that binds to the CD14 receptor expressed on the surface of monocytes and macrophages. It is used for the identification and enumeration of these cell populations in flow cytometry applications.

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Lab products found in correlation

Mouse anti human cd4 pe, by BD (2 mentions) Indomethacin, by Merck Group (2 mentions) Il 23, by Thermo Fisher Scientific (2 mentions) Forskolin, by Merck Group (2 mentions) Mouse anti human cd28, by BD (2 mentions) Ultrapure tlr4 agonist salmonella minnesota lps, by InvivoGen (2 mentions) Mouse anti human cd4 pe cy7, by Thermo Fisher Scientific (2 mentions) Mouse anti human cd45ra fitc, by Thermo Fisher Scientific (2 mentions) Interleukin 4 (il 4), by R&D Systems (2 mentions) Ifn γ, by R&D Systems (2 mentions) Annexin 5 pi staining kit, by BD (2 mentions) Rhtnf α, by Thermo Fisher Scientific (1 mentions) Rhgm csf, by Thermo Fisher Scientific (1 mentions) Interleukin 2 (il 2), by R&D Systems (1 mentions) Rh il 6, by Immunotools (1 mentions) Recombinant human gm csf, by Thermo Fisher Scientific (1 mentions) Interleukin 2 (il 2), by BD (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Recombinant human il 1β, by Immunotools (1 mentions)

Spelling variants of this product

CD14-PE (35 citations) Anti-CD14-PE (8 citations) Anti-mouse Cy5 (5 citations) CD14-PE-Cy5 (4 citations)

2 protocols using mouse anti human cd14 pe cy5

Cytokine-Induced Monocyte Activation

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All reagents were endotoxin free. rhGM-CSF and rhTNF-α were from PeproTech (Rocky Hill, NJ, USA); IL-4 and IFN-γ were from R&D Systems Europe (Oxford, United Kingdom); Ultrapure TLR4 agonist (Salmonella Minnesota LPS) was from InvivoGen (San Diego, CA, USA); rhIL-1β and rhIL-6 were from ImmunoTools (Friesoythe, Germany); IL-23 was from eBioscience (San Diego, CA, USA); and PGE₂, indomethacin, and forskolin were from Sigma-Aldrich (Dorset, United Kingdom). Mouse anti-human CD4-PE was from BD Biosciences (Oxford, United Kingdom); Mouse anti-human CD4-PE-Cy7, mouse anti-human CD45RA-FITC, mouse anti-human CD14-PE-Cy5.5, and matching isotype controls were from eBioscience. For CD4 activation, mouse anti-human CD28 was obtained from BD Biosciences, IL-2 from R&D Systems Europe, and CD3 (OKT3) was produced in-house. The annexin V/PI staining kit was obtained from BD Biosciences.

Malecka A., Wang Q., Shah S., Sutavani R.V., Spendlove I., Ramage J.M., Greensmith J., Franks H.A., Gough M.J., Saalbach A., Patel P.M, & Jackson A.M. (2016). Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation. Journal of Leukocyte Biology, 100(2), 381-389.

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Human Dendritic Cell Differentiation

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All reagents were endotoxin-free. Recombinant human GM-CSF and TNFα were from PeproTech, Rocky Hill, NJ; IL-4 and IFNγ were from R&D Systems Europe, Oxford, U.K.; Ultrapure TLR4-agonist (Salmonella Minnesota LPS) was from InvivoGen (San Diego, CA); Recombinant human IL-1β and IL-6 were from Immunotools, Friesoythe, Germany; IL-23 was from eBioscience (San Diego, CA); PGE 2 , Indomethacin and forskolin were from Sigma-Aldrich (Dorset, U.K.). Mouse anti-human CD4-PE was from BD Biosciences (Oxford, U.K.); Mouse anti-human CD4-PECy7, mouse anti-human CD45RA-FITC, mouse anti-human CD14-Pe-Cy5.5 and matching isotype controls were from eBioscience (San Diego, CA). For CD4 activation, mouse anti-human CD28 was obtained from BD Biosciences, IL-2 (R&D systems), and CD3 (OKT3) was produced in-house. AnnexinV/PI staining kit was obtained from BD Bioscience (Oxford, UK).

Langosch S., Wehner R., Malecka A., Franks H.A., Schäkel K., Bachmann M., Jackson A.M, & Schmitz M. (2016). Impact of p38 mitogen-activated protein kinase inhibition on immunostimulatory properties of human 6-sulfo LacNAc dendritic cells. Immunobiology, 221(2).

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