Quantikine elisa tgfβ1 immunoassay kit

Manufactured by R&D Systems

Sourced in United States

The Quantikine ELISA TGFβ1‐immunoassay kit is a quantitative sandwich enzyme immunoassay designed to measure transforming growth factor beta 1 (TGF‐β1) levels in cell culture supernates, serum, plasma, and other biological fluids. The kit utilizes a microplate pre‐coated with a monoclonal antibody specific for TGF‐β1.

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Lab products found in correlation

Sample activation kit 1, by R&D Systems (1 mentions)

Close spelling variants of this product

Quantikine ELISA Immunoassay kit Quantikine Immunoassay kits Quantikine ELISA kit ELISA immunoassay kit Quantikine Immunoassays Quantikine ELISA Quantikine kits Quantikines ELISAs

2 protocols using quantikine elisa tgfβ1 immunoassay kit

Serum TGFβ1 Quantification Protocol

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Serum TGFβ levels were measured according to the manufacturer's instruction using the Quantikine ELISA TGFβ1‐immunoassay kit (R&D Systems, Minneapolis, MN, USA).

Seino K., Nakano T., Tanaka T., Hozumi Y., Topham M.K., Goto K, & Iseki K. (2023). Ablation of DGKα facilitates α‐smooth muscle actin expression via the Smad and PKCδ signaling pathways during the acute phase of CCl4 ‐induced hepatic injury. FEBS Open Bio, 14(2), 300-308.

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Quantification of TGF-β1 in BAL Fluid

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TGF-β1 was quantified in BAL supernatant fluid utilizing a Quantikine ELISA TGF-β1 Immunoassay kit (R&D Systems #BD100B) according to manufacturer’s instructions and utilizing a Sample Activation Kit 1 (R&D Systems #DY010) and Human TGF-β1 Controls (R&D Systems #QC01-1). Each animal’s necropsy BAL supernatant was compared to baseline samples, and log² fold change was calculated.

Fears A.C., Beddingfield B.J., Chirichella N.R., Slisarenko N., Killeen S.Z., Redmann R.K., Goff K., Spencer S., Picou B., Golden N., Midkiff C.C., Bush D.J., Branco L.M., Boisen M.L., Gao H., Montefiori D.C., Blair R.V., Doyle-Meyers L.A., Russell-Lodrigue K., Maness N.J, & Roy C.J. (2022). Exposure modality influences viral kinetics but not respiratory outcome of COVID-19 in multiple nonhuman primate species. PLoS Pathogens, 18(7), e1010618.

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