Fp1494001kt

Manufactured by PerkinElmer

FP1494001KT is a laboratory equipment product manufactured by PerkinElmer. It is designed to perform specific functions in a laboratory setting. The core function of this product is to provide the necessary capabilities for laboratory tasks, but without further details on its intended use or interpretation of its capabilities.

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Lab products found in correlation

Fp1495001kt, by PerkinElmer (2 mentions) Fp1496001kt, by PerkinElmer (2 mentions) Fp1488001kt, by PerkinElmer (2 mentions) Fp1487001kt, by PerkinElmer (2 mentions) Antigenfix, by Diapath (2 mentions)

2 protocols using fp1494001kt

Multiplex RNA Visualization in Tissue Sections

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Experiments were performed using the RNAScope Multiplex Fluorescent V2 Assay kit (ACDBio 323100). Probes targeting intronic regions for Hs-Cd5l (ACDBio 850511), Mfa-Cd5l (ACDBio 873211), Mm-Cd5l (ACDBio 573271), Mm-Flt3 (ACDBio 487861), Mm-Xcr1 (ACDBio 562371), Mm-Mafb (ACDBio 438531) and Mm-Mgl2-O1 (ACDBio 822901) were custom-designed and synthesized. They were then labelled with TSA opal 520 (PerkinElmer FP1487001KT), TSA opal 540 (PerkinElmer FP1494001KT), TSA opal 570 (PerkinElmer FP1488001KT), TSA opal 620 (PerkinElmer FP1495001KT) or TSA opal 650 (PerkinElmer FP1496001KT). Tissues were fixed for 16 hours in AntigenFix (Diapath P0016), dehydrated and embedded in OCT as described above. Slices were pre-treated with hydrogen peroxide for 10 min and protease III for 20 min. The recommended Antigen retrieval step was not performed in order to preserve epitope integrity. Probes were hybridized and amplified according to the manufacturer’s instructions. Slides were then stained for protein markers as described above.

Guilliams M., Bonnardel J., Haest B., Vanderborght B., Wagner C., Remmerie A., Bujko A., Martens L., Thoné T., Browaeys R., De Ponti F.F., Vanneste B., Zwicker C., Svedberg F.R., Vanhalewyn T., Gonçalves A., Lippens S., Devriendt B., Cox E., Ferrero G., Wittamer V., Willaert A., Kaptein S.J., Neyts J., Dallmeier K., Geldhof P., Casaert S., Deplancke B., ten Dijke P., Hoorens A., Vanlander A., Berrevoet F., Van Nieuwenhove Y., Saeys Y., Saelens W., Van Vlierberghe H., Devisscher L, & Scott C.L. (2022). Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches. Cell, 185(2), 379-396.e38.

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Multiplex RNA Profiling in Tissue Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Experiments were performed using the RNAScope Multiplex Fluorescent V2 Assay kit (ACDBio 323100). Probes targeting intronic regions for Hs-Cd5l (ACDBio 850511), Mfa-Cd5l (ACDBio 873211), Mm-Cd5l (ACDBio 573271), Mm-Flt3 (ACDBio 487861), Mm-Xcr1 (ACDBio 562371), Mm-Mafb (ACDBio 438531) and Mm-Mgl2-O1 (ACDBio 822901) were customdesigned and synthesized. They were then labelled with TSA opal 520 (PerkinElmer FP1487001KT), TSA opal 540 (PerkinElmer FP1494001KT), TSA opal 570 (PerkinElmer FP1488001KT), TSA opal 620 (PerkinElmer FP1495001KT) or TSA opal 650 (PerkinElmer FP1496001KT). Tissues were fixed for 16 hours in AntigenFix (Diapath P0016), dehydrated and embedded in OCT as described above. Slices were pre-treated with hydrogen peroxide for 10 min and protease III for 20 min. The recommended Antigen retrieval step was not performed in order to preserve epitope integrity. Probes were hybridized and amplified according to the manufacturer's instructions. Slides were then stained for protein markers as described above.

Guilliams M., Bonnardel J., Haest B., Vanderborght B., Bujko A., Martens L., Thoné T., Browaeys R., Ponti F.F.D., Remmerie A., Wagner C., Vanneste B., Zwicker C., Vanhalewyn T., Gonçalves A., Lippens S., Devriendt B., Cox E., Ferrero G., Wittamer V., Willaert A., Kaptein S.J.F., Neyts J., Dallmeier K., Geldhof P., Casaert S., Deplancke B., Dijke P.t., Hoorens A., Vanlander A., Berrevoet F., Nieuwenhove Y.V., Saeys Y., Saelens W., Vlierberghe H.V., Devisscher L., & Scott C.L. (2021). Spatial proteogenomics reveals distinct and evolutionarily-conserved hepatic macrophage niches.

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