The murine fibroblast line L-929 were purchased from Xiehe Hospital. The antibodies used in this study were as follows: anti-α-SMA (1:1000 for western blotting, 1:50,000 for immunohistochemistry [IHC], 1:50 for immunofluorescence [IF] ab240654, Abcam, UK), anti-COL1A1 (1:1000 for western blotting, 1:100 for IHC, 1:2,000 for IF, ab270993, Abcam, UK), anti-mouse fibronectin (FN) (1:1000 for western blotting, ab268020, 1:50 for IF Abcam, UK), anti-NOX4 (1:2000 for western blotting, 1:500 for IHC, 14347-1-AP, Proteintech, USA), anti-NLRP3 (1:2000 for western blotting, 1:200 for IHC, 68102-1-Ig, Proteintech, USA), anti-p38 (1:500 for western blotting, 1:1000 for IHC, WLH3870, Wanleibio, China), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:1000 for western blotting, ab8245, Abcam, UK), anti-beta-actin (β-actin) (1:1000 for western blotting, ab8226, Abcam, UK), anti-caspase-1 (1:500 for western blotting, GB11383, Servicebio, China), anti-ASC (1:1000 for western blotting, ab309497, Abcam, UK), anti-IL-1β (1:1000 for western blotting, ab254360, Abcam, UK), anti-mouse IL-18 (1:2000 for western blotting, GB114098, Servicebio, China), and anti-phospho-p38 (p-p38) (1:1000 for western blotting, 28796-1-AP, Proteintech, USA).
Ab309497
Manufactured by Abcam
Sourced in United Kingdom
Ab309497 is a primary antibody product manufactured by Abcam. It is designed for use in various laboratory techniques, but no further details about its specific function or intended use are provided to maintain an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Ab254360, by Abcam (1 mentions)
Ab270993, by Abcam (1 mentions)
Ab268020, by Abcam (1 mentions)
Ab240654, by Abcam (1 mentions)
Ab8226, by Abcam (1 mentions)
Gb11383, by Wuhan Servicebio Technology (1 mentions)
Ab8245, by Abcam (1 mentions)
Ab207802, by Abcam (1 mentions)
Ab270449, by Abcam (1 mentions)
Ab315084, by Abcam (1 mentions)
Ab213262, by Abcam (1 mentions)
Detergent compatible protein assay kit, by Bio-Rad (1 mentions)
Nbp2 24683, by Novus Biologicals (1 mentions)
2 protocols using ab309497
1
Murine Fibroblast Immunological Profiling
2
NLRP3 Inflammasome Signaling in SAH
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Western blot was performed according to our previously protocols [16 (link)]. Briefly, cerebral cortical tissue was harvested from the injured side of SAH, then homogenized and centrifuged. The detergent compatible protein assay kit (Bio-Rad, Hercules, California) was used to determine the protein content. Equal amounts of protein (30 μg) were loaded into the wells of the SDS-PAGE gel. After electrophoresis, the protein was transferred to a nitrocellulose membrane. The membrane was blocked for 2 h using a nonfat dry milk buffer and incubated overnight at 4 °C with the primary antibodies. The following primary antibodies were used: anti-STING (1 : 1000, NBP2-24683, Novus), anti-NLRP3 (1 : 1000, Abcam, ab270449), anti-ASC (1 : 1000, Abcam, ab309497), anti-caspase-1 (1 : 1000, Abcam, ab207802), anti-IL-1β (1 : 1000, Abcam, ab315084), anti-β-actin (1 : 1000, Abcam, ab213262). The membrane was incubated for 1 h with secondary antibodies at room temperature. The membrane was exposed to X-ray film, and band densities were analyzed using Image J software. To facilitate comparisons between groups, band density values were normalized to the mean value for the sham group.
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